Alexa Fluor 488 anti-Oct4 Oct3 Antibody anti-Oct4

Pricing & Availability

Clone: 3A2A20 (See other available formats)
Regulatory Status: RUO
Other Names: POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype: Mouse IgG2b, κ
Ave. Rating: Submit a Review
Product Citations: publications

3A2A20_AF488_OCT4_Antibody_FC_062413 — Human teratocarcinoma cell line NCCIT cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with anti-human OCT4 (clone 3A2A20) Alexa Fluor® 488 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).

Compare all formats See Alexa Fluor® 488 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

653705

25 tests

171 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

653706

100 tests

382 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Oct4 recombinant protein (1-141aa).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2562250 (BioLegend Cat. No. 653705)
AB_2562250 (BioLegend Cat. No. 653706)

Antigen Details

Structure: Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution: Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction: SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References: 1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271.
Gene ID: 5460 View all products for this Gene ID
UniProt: View information about Oct4 on UniProt.org

Documentation

Reviews

Review This Product

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Oct4 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Oct4 (Oct3)	3A2A20	WB,ICC,ChIP
PE anti-Oct4 (Oct3)	3A2A20	ICFC
Alexa Fluor® 488 anti-Oct4 (Oct3)	3A2A20	ICFC
Brilliant Violet 421™ anti-Oct4 (Oct3)	3A2A20	ICC,ICFC
Alexa Fluor® 647 anti-Oct4 (Oct3)	3A2A20	ICC,ICFC

Certificate of Analysis

Customers Also Purchased

PE anti-human TRA-1-60-R

Clone: TRA-1-60-R

PE anti-human CD14

Clone: M5E2

Alexa Fluor® 488 anti-human/mouse SSEA-3

Clone: MC-631

APC anti-human CD45

Clone: HI30

Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.