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Alexa Fluor® 647 anti-Oct4 (Oct3) Antibody

Pricing & Availability
Clone
3A2A20 (See other available formats)
Regulatory Status
RUO
Other Names
POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_3A2A20_A647_Oct4_Oct3_Antibody_ICC_080219
NCCIT pluripotent embryonal carcinoma cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Oct4 (Oct3) (clone 3A2A20) Alexa Fluor® 647 (red) in 5% FBS overnight at 4°C and Alexa Fluor® 488 Phalloidin (green) for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 1_3A2A20_A647_Oct4_Oct3_Antibody_ICC_080219
    NCCIT pluripotent embryonal carcinoma cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Oct4 (Oct3) (clone 3A2A20) Alexa Fluor® 647 (red) in 5% FBS overnight at 4°C and Alexa Fluor® 488 Phalloidin (green) for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 2_3A2A20_A647_Oct4_Antibody_ICFC_060617
    Human teratocarcinoma cell line NCCIT cells were fixed and permeabilized with BioLegend’s FOXP3 Fix/Perm Buffer Set, and then stained with anti-Oct4 (clone 3A2A20) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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653710 100 µg 347 CHF
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Description

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Oct4 recombinant protein (1-141aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Dong T, et al. 2021. Stem Cell Research. 51:102196. PubMed
  2. Ervin EH, et al. 2019. Stem Cell Res Ther. 10:43. PubMed
RRID
AB_2566689 (BioLegend Cat. No. 653710)

Antigen Details

Structure
Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution

Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction
SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area
Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271.

Gene ID
5460 View all products for this Gene ID
UniProt
View information about Oct4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.02.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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