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Alexa Fluor® 488 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Alexa Fluor® 488, Streptavidin-AF488, SAV
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Product Citations
publications
Strepavidin_A488_Antibody_FC_013114
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 488.
  • Strepavidin_A488_Antibody_FC_013114
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 488.
See Alexa Fluor® 488 spectral data
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405235 100 µg 206 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 488 is useful for detecting biotinylated antibodies. The excitation of Alexa Fluor® 488 by 488 nm laser light induces a light emission maximum of 519 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin was conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Alexa Fluor® 488 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 488 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-Alexa Fluor® 488 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 488 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed
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  5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
  6. Yamakawa N, et al. 2012. Int Immunol. PubMed
  7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
  8. Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
  9. Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
  10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
  11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
  12. Reading JL, 2013. J. immunol. 190:4542. Pubmed.
  13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
Product Citations
  1. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  2. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  3. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  4. Churchill MJ, et al. 2022. J Exp Med. 219:. PubMed
  5. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  6. Tran JR, et al. 2022. Nucleic Acids Res. 50:e117. PubMed
  7. Melzi E, et al. 2022. Immunity. 55:2168. PubMed
  8. Peng W, et al. 2022. Cell Mol Life Sci. 79:602. PubMed
  9. Han Y, et al. 2020. Vaccines (Basel). 8:00. PubMed
  10. Tran JR, et al. 2021. J Cell Biol. 220: . PubMed
  11. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  12. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  13. Aneas I, et al. 2021. Nat Commun. 12:6115. PubMed
  14. Tomić S, et al. 2016. Sci Rep. 6:31618. PubMed
  15. Tomić S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.08.040. PubMed
  16. Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed
  17. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  18. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  19. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  20. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  21. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  22. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  23. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  24. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  25. Lederer K, et al. 2022. Cell. . PubMed
  26. Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed
  27. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  28. Tichy ED, et al. 2021. Cell Rep. 35:109098. PubMed
Go To Top Version: 5    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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