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Alexa Fluor® 594 anti-Histone H3 (C-terminus) Antibody

Pricing & Availability
Clone
1B1-B2 (See other available formats)
Regulatory Status
RUO
Other Names
Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
1B1-B2_A594_Histone_H3_Antibody_1_012122
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red), and 5 µg/ml of anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 488 (green) at 4°C overnight. Nuclei were counterstained with DAPI (purple). The image was captured by 10X objective.
  • 1B1-B2_A594_Histone_H3_Antibody_1_012122
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red), and 5 µg/ml of anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 488 (green) at 4°C overnight. Nuclei were counterstained with DAPI (purple). The image was captured by 10X objective.
  • 1B1-B2_A594_Histone_H3_Antibody_2_012122
    TE-71 cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red) at 4°C overnight. The following day the cells were stained with Flash Phalloidin™ NIR 647 (green) for 20 minutes in room temperature. Nuclei were counterstained with DAPI (purple). The image was captured with a 60X objective.
  • 1B1-B2_A594_Histone_H3_Antibody_3_012122
    Human paraffin-embedded breast cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH 7.4) at 95°C for 40 minutes. The tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (purple). The image was captured with a 10X objective.
  • 1B1-B2_A594_Histone_H3_Antibody_4_012122
    Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH 7.4) at 95°C for 40 minutes. The tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (purple). The image was captured with a 10X objective.
  • 1B1-B2_A594_Histone_H3_Antibody_5_012122
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH 7.4) at 95°C for 40 minutes. The tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (purple). The image was captured with a 10X objective.
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819405 25 µg 147 CHF
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819406 100 µg 370 CHF
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide; may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.25 - 5 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.25 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Reactivity to Drosophila was only verified with the purified format.

RRID
AB_2715800 (BioLegend Cat. No. 819405)
AB_2715800 (BioLegend Cat. No. 819406)

Antigen Details

Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 4    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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