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Purified anti-Histone H3 (C-terminus) Antibody

Pricing & Availability
Clone
1B1-B2 (See other available formats)
Regulatory Status
RUO
Other Names
Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
1_1B1-B2_Purified_Histone_H3_CO2_Terminus_Antibody_IHC_030415.jpg
IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_1B1-B2_Purified_Histone_H3_CO2_Terminus_Antibody_IHC_030415.jpg
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_1B1-B2_PURE_a-HistoneH3_IHC_Antibody_2_043018.png
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_1B1-B2_PURE_a-HistoneH3_IHC_Antibody_3_043018.png
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_1B1-B2_PURE_Histone_H3_Antibody_4_WB_102519_updated
    Western blot of purified anti-Histone H3 (C-terminus) antibody (clone 1B1-B2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate; Lane 5: 10µg of Drosophila head lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5_1B1-B2_PURE_Histone_H3_Antibody_2_WB_051519
    Western blot of purified anti-Histone H3 (C-terminus) antibody (clone 1B1-B2). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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819411 25 µg 89 CHF
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819412 100 µg 270 CHF
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation
50 mM sodium citrate, 150mM sodium chloride, pH 5.8 with stabilizer
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration of 1.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.01 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 0.01 - 0.2 µg/mL in human, mouse, and rat and 0.1 - 0.5 µg/mL in Drosophila.

Product Citations
  1. Lu H, et al. 2022. Nucleic Acids Res. 50:5635. PubMed
  2. Liu PS, et al. 2023. Nat Immunol. 24:452. PubMed
  3. Reers AB, et al. 2023. Epigenetics Chromatin. 16:25. PubMed
  4. Bado IL, et al. 2021. Developmental Cell. 56(8):1100-1117.e9. PubMed
RRID
AB_2820128 (BioLegend Cat. No. 819411)
AB_2820128 (BioLegend Cat. No. 819412)

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Nucleus

Function
Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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