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Alexa Fluor® 594 anti-human CD44 variant 9 (CD44v9) Antibody

Pricing & Availability
Clone
RV3 (See other available formats)
Regulatory Status
RUO
Other Names
CD44 variant 9, CD44 variant isoform 9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RV3_A594_CD44v9_Antibody_100918
Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44v9 (clone RV3) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • RV3_A594_CD44v9_Antibody_100918
    Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44v9 (clone RV3) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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394405 25 µg 136 CHF
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394406 100 µg 347 CHF
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Description

CD44 isoform 9, also known as CD44v9, is a CD44 isofom consisting of exons 1-5, 13 (v9) and 15 to 19, product of alternative splicing. CD44v9 is expressed by cancer stem cells, epithelial-type carcinomas, subsets of monocytes and subsets of lymphocytes; have a role in tumor initiation, maintenance and metastasis of cancer cells. CD44v9 interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells, and is a potential predictive marker for recurrence of some cancers and a potential therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD44v8-10 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kakehashi, A., et al. 2016. Cancer Sci. 107: 609. (IHC-P)
  2. Aso T, et al. 2015. PLoS One. 10: e0116596. (IHC-P)
  3. Yoshikawa M, et al. 2013. Cancer Res. 73: 1855. (IHC-F)
  4. Ishimoto T, et al. 2011. Cancer Cell. 387-400. (IHC-P)
RRID
AB_2783305 (BioLegend Cat. No. 394405)
AB_2783305 (BioLegend Cat. No. 394406)

Antigen Details

Distribution

Cancer stem cells, epithelial-type carcinomas, subset of monocytes, subset of lymphocytes.

Function
Role in tumor initiation and maintenance and metastasis of cancer cells, Interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells.
Cell Type
Epithelial cells, Lymphocytes, Monocytes, Neutrophils
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Hagiwara M, et al. 2018. BMC Cancer. 18:113
  2. Matsumoto T, et al. 2017. Oncogenesis. 6:397
  3. Bertaux-Skeirik N, et al. 2017. J. Pathol. 242:463
  4. Kodama H, et al. 2017. Br. J. Cancer. 116:186
Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44v9 on UniProt.org
Go To Top Version: 2    Revision Date: 02.22.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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