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Alexa Fluor® 594 anti-rat IgG1 Antibody

Pricing & Availability
Clone
MRG1-58 (See other available formats)
Regulatory Status
RUO
Other Names
Imunoglobulin G1
Isotype
Mouse IgG
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Product Citations
publications
1-MRG1-58_A694_IgG1_Antibody_IHC_110917
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Purified CD8b.2 (clone 53-5.8) at 4°C overnight. The staining was followed by 5 µg/ml anti-Rat IgG1 (clone MRG1-58) Alexa Fluor® 594 (red) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488(green) at room temperature for 2 hours. The image was captured by 10X objective.
  • 1-MRG1-58_A694_IgG1_Antibody_IHC_110917
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Purified CD8b.2 (clone 53-5.8) at 4°C overnight. The staining was followed by 5 µg/ml anti-Rat IgG1 (clone MRG1-58) Alexa Fluor® 594 (red) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488(green) at room temperature for 2 hours. The image was captured by 10X objective.
  • 2-MRG1-58_A594_IgG1_Antibody_ICC_2_062123
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with MeOH for 10 minutes, and blocked with 5% FBS for 1 hour. Cells were then stained with 5.0 µg/mL of purified rat anti-NR1D2 antibody (Cat. No. 689402) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG1 (clone MRG1-58) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801). The images were captured with a 40X objective. Scale bar = 50 µm
Compare all formats See Alexa Fluor® 594 spectral data
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407409 25 µg 112 CHF
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407410 100 µg 218 CHF
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Description

The MRG1-58 monoclonal antibody reacts with rat immunoglobulin G1 (IgG1) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG1-58 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2721399 (BioLegend Cat. No. 407409)
AB_2721399 (BioLegend Cat. No. 407410)

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Go To Top Version: 2    Revision Date: 06.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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