- Clone
- MRG2a-83 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2a
- Isotype
- Mouse IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MRG2a-85_A647_IgG2b_Antibody_FC_071218 MRG2a-85_A647_IgG2b_Antibody_FC_071218](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MRG2a-83_A647_IgG2a_Antibody_FC_071218.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 647 . -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD8a (clone 53-6.7) purified and 10 µg/ml of CD20 (clone SA275A11) Brilliant Violet 421™ (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG2a (Clone MRG2a-83) Alexa Fluor® 647 (red) for 2 hours at room temperature. The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
407511 | 25 µg | 89 CHF | ||||
407512 | 100 µg | 194 CHF |
The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mixed rat Igs
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Inoue S, et al. 2006. Cancer Research 66:7741.
- Product Citations
-
- RRID
-
AB_2716139 (BioLegend Cat. No. 407511)
AB_2716140 (BioLegend Cat. No. 407512)
Other Formats
View All IgG2a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-rat IgG2a | MRG2a-83 | ELISA,FC |
Biotin anti-rat IgG2a | MRG2a-83 | ELISA,FC |
FITC anti-rat IgG2a | MRG2a-83 | FC |
PE anti-rat IgG2a | MRG2a-83 | FC |
Alexa Fluor® 594 anti-rat IgG2a | MRG2a-83 | IHC-F,ICC |
Alexa Fluor® 647 anti-rat IgG2a | MRG2a-83 | FC,IHC-F |
Alexa Fluor® 488 anti-rat IgG2a | MRG2a-83 | FC,IHC-F |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-rat IgG2a
-
Biotin anti-rat IgG2a
-
FITC anti-rat IgG2a
-
PE anti-rat IgG2a
C57BL/6 mouse splenocytes were stained with purified CD19 fo... -
Alexa Fluor® 594 anti-rat IgG2a
C57BL/6 frozen mouse spleen section was fixed with 4% parafo... HeLa cells were fixed with 4% paraformaldehyde for 10 minute... -
Alexa Fluor® 647 anti-rat IgG2a
C57BL/6 mouse splenocytes were stained with purified CD19 (c... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 488 anti-rat IgG2a
C57BL/6 mouse splenocytes were stained with purified anti-mo... C57BL/6 mouse frozen spleen section was fixed with 4% parafo...
Follow Us