Alexa Fluor 594 anti-TAL1 Antibody anti-TAL1

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Clone: 4G8C07 (See other available formats)
Regulatory Status: RUO
Other Names: T-cell acute lymphocytic leukemia 1, Class A basic helix-loop-helix protein 17 (bHLHa17), T-cell leukemia/lymphoma protein 5 (TCL5), SCL
Isotype: Mouse IgG1, κ
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Product Citations: publications

4G8C07_A594_TAL1_Antibody_1_ICC_042419 — Jurkat cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1 µg/ml Alexa Fluor® 594 anti-human TAL1 (clone 4G8C07) antibody (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by a 60X objective.

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681004

100 µg

405 CHF

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Description

TAL1, also known as SCL, is a class I basic helix-loop-helix (bHLH) transcription factor. TAL1 is essential for the establishment of hematopoietic stem cells during embryogenesis, and regulating proliferation and differentiation of multiple hematopoeitic cell lineages, such as erythrocytes, megakaryocytes, mast cells, and eosinoohils. Tal1-null mice are embryonic lethal due to the failure of yolk sac hematopoiesis and vascular defects. In hematopoietic cells, TAL1 forms a multiprotein complex with other transcription factors, which include the E protein TCF3, LMO2, LDB1, and GATA family members. The large protein complex containing TAL1 binds to specific cis-regulatory elements encoding the consensus sequence AACAGATGGT and regulates expression of target genes. TAL1 is not expressed in normal lymphoid cells, but ectopic expression of TAL1 in T-cells leads to T-cell acute lymphocytic leukemia (T-ALL).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human TAL1 recombinant protein (1-263 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID: AB_2572200 (BioLegend Cat. No. 681004)

Antigen Details

Structure: 331 amino acids with a predicted molecular weight of 34.3 kD. Contains a helix-loop-helix (bHLH) domain that is responsible for DNA binding and protein to protein interaction.
Distribution: Nucleus.
Function: TAL1 is a bHLH transcription factor that regulates hematopoietic and vascular development. Ectopic expression of TAL1 is linked to T-cell acute lymhoblastic leukemia (T-ALL).
Interaction: Forms heterodimers with TCF3. Interacts with ETS1, LMO2, and DRG1.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Wu W, et al. 2014. Genome Res. 24:1945.
2. Kolodziej S, et al. 2014. Nat. Commun. 5:3995.
3. Palii CG, et al. 2014. Cell Stem Cell 14:644.
4. Yun WJ, et al. 2014. Nucleic Acids Res. 42:4283.
5. Zhou Y, et al. 2013. Blood 26:4199.
6. Sanda T, et al. 2012. Cancer Cell 22:209.
Gene ID: 6886 View all products for this Gene ID
UniProt: View information about TAL1 on UniProt.org

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Immunocytochemistry Staining Protocol

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Other Formats

View All TAL1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-TAL1	4G8C07	WB,ICC,ICFC
Alexa Fluor® 594 anti-TAL1	4G8C07	ICC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 04.24.2019

For Research Use Only. Not for diagnostic or therapeutic use.

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