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Purified anti-TAL1 Antibody

Pricing & Availability
Clone
4G8C07 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell acute lymphocytic leukemia 1, Class A basic helix-loop-helix protein 17 (bHLHa17), T-cell leukemia/lymphoma protein 5 (TCL5), SCL
Isotype
Mouse IgG1, κ
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Product Citations
publications
4G8C07_PURE_TAL1_Antibody_1_WB_093015
Total cell lysate from human Jurkat cells (lane 1), 293T cells (lane 2), and mouse thymus (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TAL1 (clone 4G8C07) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 4G8C07_PURE_TAL1_Antibody_1_WB_093015
    Total cell lysate from human Jurkat cells (lane 1), 293T cells (lane 2), and mouse thymus (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TAL1 (clone 4G8C07) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 4G8C07_PURE_TAL1_Antibody_2_ICC_042419
    Jurkat cells were stained with purified anti-TAL1 (clone 4G8C07) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-mouse IgG (red) antibody. Nuclei were stained with DAPI (blue).
  • 4G8C07_PURE_TAL1_Antibody_3_ICFC_062123
    Daudi cells (negative control, open histogram) or Jurkat cells (positive control, filled histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with purified anti-TAL1 (clone 4G8C07), or purified mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 401401), followed by PE goat anti-mouse IgG (Cat. No. 405307).
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681001 25 µg 112 CHF
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681002 100 µg 288 CHF
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Description

TAL1, also known as SCL, is a class I basic helix-loop-helix (bHLH) transcription factor. TAL1 is essential for the establishment of hematopoietic stem cells during embryogenesis, and regulating proliferation and differentiation of multiple hematopoeitic cell lineages, such as erythrocytes, megakaryocytes, mast cells, and eosinoohils. Tal1-null mice are embryonic lethal due to the failure of yolk sac hematopoiesis and vascular defects. In hematopoietic cells, TAL1 forms a multiprotein complex with other transcription factors, which include the E protein TCF3, LMO2, LDB1, and GATA family members. The large protein complex containing TAL1 binds to specific cis-regulatory elements encoding the consensus sequence AACAGATGGT and regulates expression of target genes. TAL1 is not expressed in normal lymphoid cells, but ectopic expression of TAL1 in T-cells leads to T-cell acute lymphocytic leukemia (T-ALL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TAL1 recombinant protein (1-263 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. For flow cytometric staining using our True-Phos™ Perm Buffer in Whole Blood Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566441 (BioLegend Cat. No. 681001)
AB_2566441 (BioLegend Cat. No. 681002)

Antigen Details

Structure
331 amino acids with a predicted molecular weight of 34.3 kD. Contains a helix-loop-helix (bHLH) domain that is responsible for DNA binding and protein to protein interaction.
Distribution

Nucleus.

Function
TAL1 is a bHLH transcription factor that regulates hematopoietic and vascular development. Ectopic expression of TAL1 is linked to T-cell acute lymhoblastic leukemia (T-ALL).
Interaction
Forms heterodimers with TCF3. Interacts with ETS1, LMO2, and DRG1.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wu W, et al. 2014. Genome Res. 24:1945.
2. Kolodziej S, et al. 2014. Nat. Commun. 5:3995.
3. Palii CG, et al. 2014. Cell Stem Cell 14:644.
4. Yun WJ, et al. 2014. Nucleic Acids Res. 42:4283.
5. Zhou Y, et al. 2013. Blood 26:4199.
6. Sanda T, et al. 2012. Cancer Cell 22:209.

Gene ID
6886 View all products for this Gene ID
UniProt
View information about TAL1 on UniProt.org
Go To Top Version: 3    Revision Date: 06.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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