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Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2) Antibody

Pricing & Availability
Clone
A20145B (See other available formats)
Regulatory Status
RUO
Other Names
HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, oncogene NGL, neuroblastoma- or glioblastoma-derived, HER-2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A20145B_A647_CD340_Antibody_071823
Human breast cancer cell line (SK-BR-3) was stained with anti-CD340 (ErbB2/HER-2) (clone A20145B) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 (open histogram).
  • A20145B_A647_CD340_Antibody_071823
    Human breast cancer cell line (SK-BR-3) was stained with anti-CD340 (ErbB2/HER-2) (clone A20145B) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 (open histogram).
See Alexa Fluor® 647 spectral data
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616153 25 tests 140 CHF
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616154 100 tests 320 CHF
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Description

CD340 is the protein HER2, a member of the epidermal growth factor receptor (EGFR) family of cell membrane tyrosine kinases, and is encoded by the gene ErbB2. Unlike other EGFR family members, CD340 contains no ligand binding domain and has no known ligands. Instead, this protein interacts with other EGF receptor family members to form a heterodimer, stabilizing ligand binding, and enhancing kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human ErbB2, N-terminus
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This product is not recommended for use in IHC-P.

RRID
AB_3097216 (BioLegend Cat. No. 616153)
AB_3097216 (BioLegend Cat. No. 616154)

Antigen Details

Structure
CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kDa.
Distribution

Expressed on the cell surface of many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, glioblastoma, some c-ALL blasts and bone marrow MSCs.

Function
Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
Interaction
CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin-1, JAK2, PAK1, FAK, and β-catenin, among others.
Ligand/Receptor
Despite being a member of the epidermal growth factor receptor (EGFR) family, no ligand of CD340 has been identified.
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukocyte Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org
Go To Top Version: 1    Revision Date: 07.18.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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