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Alexa Fluor® 647 anti-mouse CD169 (Siglec-1) Antibody

Pricing & Availability
Clone
3D6.112 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_3D6.112_A647_CD169_Antibody_FC_1_060914.jpg
C57BL/6 mouse bone marrow cells were stained with F4/80 PE, Ly-6G FITC and CD169 (clone 3D6.112) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data were analyzed by gating on Ly-6Gdim/neg cell population.
  • A_3D6.112_A647_CD169_Antibody_FC_1_060914.jpg
    C57BL/6 mouse bone marrow cells were stained with F4/80 PE, Ly-6G FITC and CD169 (clone 3D6.112) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data were analyzed by gating on Ly-6Gdim/neg cell population.
  • B_3D6.112_A647_CD169_Antibody_FC_2_060914
  • C_3D6.112_A647_CD169_IHCF_120817
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/mL of CD169 (clone 3D6.112) Alexa Fluor® 647 (red), 2.5 µg/mL of B220 (clone RA3‐6B2) Alexa Fluor® 488 (blue), and 2.5 µg/mL of CD3 (clone 17A2) Alexa Fluor® 594 (green) overnight at 4˚C. The image was captured by 10X objective.
  • D_Still-image_0408-1_10X-1_1
    Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD3 (clone 17A2) Alexa Fluor® 594 (red), CD169 (Siglec-1)(clone 3D6.112) Alexa Fluor® 647 (green) both at 5 µg/mL, optically cleared, then analyzed at 225 μm imaging depth on a confocal microscope. Watch the video.
  • E_3D6dot112_A647_CD169_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (1%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 594 at 5 µg/mL (yellow), anti-mouse CD169 (Siglec-1) Antibody (clone 3D6.112) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
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Cat # Size Price Quantity Check Availability Save
142407 25 µg 171 CHF
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142408 100 µg 382 CHF
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Description

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8+ T cells and invariant NK cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Native Sialoadhesin from spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume.  For immunohistochemisty on frozen tissue sections, a concentration range of 1-5 μg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
  2. Chtanova T, et al. 2008. Immunity 29:487.
  3. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Singh R, et al. 2021. Bio Protoc. 11:e3878. PubMed
  2. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  3. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  4. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  5. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  6. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  7. Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed
  8. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  9. Le Gars M, et al. 2016. J Immunol. 197: 3225 - 3232. PubMed
  10. Freeman SA, et al. 2020. Science. 367:301. PubMed
  11. Gbotosho OT, et al. 2020. Exp Hematol. 19:84. PubMed
  12. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  13. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  14. Rojo R, et al. 2019. Nat Commun. 10:3215. PubMed
  15. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
RRID
AB_2563620 (BioLegend Cat. No. 142407)
AB_2563620 (BioLegend Cat. No. 142408)

Antigen Details

Structure
Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution

Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.

Function
Adhesion.
Ligand/Receptor
PSGL-1, CD43, CD206 and CD227.
Cell Type
Macrophages
Biology Area
Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References

1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.

Gene ID
20612 View all products for this Gene ID
UniProt
View information about CD169 on UniProt.org
Go To Top Version: 4    Revision Date: 09.21.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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