Loading...

Alexa Fluor® 647 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1_BM8_A647_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 647.
  • 1_BM8_A647_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 647.
  • 2_BM8_A647_F4slash80_Antibody_2_IHCF_120320
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-mouse F4/80 (clone BM8) Alexa Fluor® 647 (red), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (blue), and anti-mouse/rat/human CD27 (clone LG.3A10) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured with a 10X objective.
  • 3_BM8_A647_F4-80_Antibody_2_092121.png
    Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD146 Antibody (clone ME-9F1) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-mouse F4/80 Antibody (clone BM8) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
123121 25 µg 147 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123122 100 µg 323 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yan M, et al. 2022. J Cell Physiol. 237:897. PubMed
  2. Lees-Shepard JB, et al. 2022. J Clin Invest. 132: . PubMed
  3. Wang L, et al. 2022. Cell Rep. 41:111462. PubMed
  4. Aljobaily N, et al. 2022. Nutrients. 15: . PubMed
  5. Rodriguez-Ruiz ME, et al. 2023. J Immunother Cancer. 11: . PubMed
  6. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  7. Li Z, et al. 2022. Nat Commun. 13:1845. PubMed
  8. Marneros AG, et al. 2020. Developmental Cell. 54(3):348-366.e5. PubMed
  9. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  10. Barkaway A, et al. 2021. Immunity. . PubMed
  11. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  12. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  13. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  14. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  15. Lütge M, et al. 2023. Nat Immunol. . PubMed
  16. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
  17. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  18. Akincilar SC, et al. 2020. Gut. . PubMed
  19. Nocera D, et al. 2016. J Immunol. 196: 2860 - 2869. PubMed
  20. Shinohara M 2015. J Immunol. 194:5595. PubMed
  21. Badenes M, et al. 2020. Mol Metab. 31:67. PubMed
  22. Gwela A, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjx050. PubMed
  23. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  24. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  25. Marneros A 2016. EMBO Mol Med. 8: 208 - 231. PubMed
  26. Chan IS, et al. 2020. J Cell Biol. :219. PubMed
  27. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  28. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  29. Luan X, et al. 2021. Biomaterials. 275:120910. PubMed
  30. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  31. Hu K, et al. 2015. PLoS One. 10: 0137123. PubMed
  32. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  33. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  34. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  35. Chen S, et al. 2021. Immunology. 163:201. PubMed
  36. Yu CR, et al. 2022. Int J Mol Sci. 23:. PubMed
  37. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  38. Dean JM, et al. 2020. Cell Rep. 33:108228. PubMed
  39. Jiang H, et al. 2016. J Immunol. 197: 2880 - 2890. PubMed
  40. Yang Y, et al. 2016. Nat Commun. 7:11385. PubMed
  41. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
  42. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  43. Jaworska K, et al. 2015. J Immunol. 194:325. PubMed
  44. Shankar J 2016. Innate Immunity. 22: 31 - 39. PubMed
  45. Galvani E, et al. 2020. Nat Commun. 11:853. PubMed
  46. Petrus P, et al. 2020. Cell Metabolism. 31(2):375-390.e11.. PubMed
  47. Dobrian A, et al. 2011. Am J Physiol Endocrinol Metab. 300:E410. PubMed
  48. Belliere J, et al. 2015. J Am Soc Nephrol. 26:1363. PubMed
  49. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  50. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
  51. Ruiz–Manzano RA, et al. 2020. Frontiers in Oncology. 0.927777778. PubMed
  52. Daemen S, et al. 2021. STAR Protoc. 2:100511. PubMed
  53. Rani GF, et al. 2021. Wellcome Open Res. 6:83. PubMed
  54. Tavares J, et al. 2013. J Exp Med. 210:905. PubMed
  55. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  56. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  57. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  58. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  59. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  60. Qiao S, et al. 2019. J Neuroinflammation. 16:4. PubMed
  61. He L, et al. 2021. Cell Rep. 37:109955. PubMed
  62. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  63. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  64. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  65. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  66. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  67. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-00654-2. PubMed
  68. Hosaka K, et al. 2020. Nat Commun. 3.030555556. PubMed
  69. Lamontagne JO, et al. 2022. Nat Commun. 13:2226. PubMed
  70. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  71. Wang J, et al. 2015. Cancer Res. 75:306. PubMed
  72. Garg BK, et al. 2019. PLoS One. 14:e0214942. PubMed
  73. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  74. Wang S, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.104. PubMed
  75. Zeid AM, et al. 2022. FASEB J. 36:e22286. PubMed
  76. Petty AJ, et al. 2021. JCI Insight. 6:. PubMed
  77. Woods PS, et al. 2020. Am J Respir Cell Mol Biol. 62:243. PubMed
  78. Shan Z, et al. 2021. eLife. 10:00. PubMed
  79. Wang Y, et al. 2020. Immunity. 53(6):1168-1181.e7. PubMed
  80. Bai WJ, et al. 2018. Front Immunol. 8:1919. PubMed
  81. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  82. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
  83. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  84. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  85. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  86. Lin YW, et al. 2018. Sci Rep. 8:834. PubMed
RRID
AB_893480 (BioLegend Cat. No. 123121)
AB_893480 (BioLegend Cat. No. 123122)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Other Formats

View All F4/80 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 BM8 FC,IHC,WB
Biotin anti-mouse F4/80 BM8 FC,IHC
FITC anti-mouse F4/80 BM8 FC
PE anti-mouse F4/80 BM8 FC
PE/Cyanine5 anti-mouse F4/80 BM8 FC
PE/Cyanine7 anti-mouse F4/80 BM8 FC
APC anti-mouse F4/80 BM8 FC
APC/Cyanine7 anti-mouse F4/80 BM8 FC
Alexa Fluor® 488 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80 BM8 FC
PerCP anti-mouse F4/80 BM8 FC
PerCP/Cyanine5.5 anti-mouse F4/80 BM8 FC
Alexa Fluor® 700 anti-mouse F4/80 BM8 FC
Brilliant Violet 421™ anti-mouse F4/80 BM8 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80 BM8 FC
Alexa Fluor® 594 anti-mouse F4/80 BM8 IHC-F
Brilliant Violet 785™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 (Maxpar® Ready) BM8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80 BM8 FC
Brilliant Violet 650™ anti-mouse F4/80 BM8 FC
Brilliant Violet 711™ anti-mouse F4/80 BM8 FC
APC/Fire™ 750 anti-mouse F4/80 BM8 FC
TotalSeq™-A0114 anti-mouse F4/80 BM8 PG
TotalSeq™-B0114 anti-mouse F4/80 BM8 PG
TotalSeq™-C0114 anti-mouse F4/80 BM8 PG
Spark YG™ 570 anti-mouse F4/80 BM8 IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80 BM8 FC
Ultra-LEAF™ Purified anti-mouse F4/80 BM8 FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark NIR™ 685 anti-mouse F4/80 BM8 FC
Spark Blue™ 550 anti-mouse F4/80 BM8 FC
Brilliant Violet 570™ anti-mouse F4/80 BM8 FC
Brilliant Violet 750™ anti-mouse F4/80 BM8 FC
PE/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Go To Top Version: 8    Revision Date: 09.21.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account