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Alexa Fluor® 647 anti-mouse Ikaros Antibody

Pricing & Availability
Clone
2A9/Ikaros (See other available formats)
Regulatory Status
RUO
Other Names
IKAROS family zinc finger 1, DNA-binding protein Ikaros
Isotype
Mouse IgG1, κ
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Product Citations
publications
2A9_AF647_Ikaros_Antibody_FC_022014
C57BL/6 mouse splenocytes were treated FOXP3 Fix/Perm Buffer Set, and then intracellularly stained with Ikaros (clone 2A9/Ikaros) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • 2A9_AF647_Ikaros_Antibody_FC_022014
    C57BL/6 mouse splenocytes were treated FOXP3 Fix/Perm Buffer Set, and then intracellularly stained with Ikaros (clone 2A9/Ikaros) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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653305 25 µg 171 CHF
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653306 100 µg 382 CHF
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Description

Ikaros family members, including Ikaros and Helios, play a role in the development of lymphocytes and B and T cells. They are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and IL-2 mediated signaling events.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal half of mouse Ikaros fused to GST.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported references (for the relevant formats) include: immunofluorescence1 and immunoprecipitation2.

Does not cross-react with human.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

View more applications data for a different format of this clone in our Scientific Poster Library.

This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes.  Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited.  In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Cobb SB, et al. 2000. Genes & Dev. 14:2146. (IF)
  2. Hahm K. et al. 1998. Genes Dev. 12:782. (IP)
  3. Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
Product Citations
  1. M H, et al. 2016. Cell Transplant. 25: 811-27. PubMed
RRID
AB_2563162 (BioLegend Cat. No. 653305)
AB_2563162 (BioLegend Cat. No. 653306)

Antigen Details

Structure
Belongs to the Ikaros C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers. 519 amino acids with predicted MW of 58 kD.
Distribution

Nucleus. Abundantly expressed in thymus, spleen and peripheral blood leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

Function
Transcription regulator of hematopoietic cell differentiation. Plays a role in the development of lymphocytes, B and T cells.
Interaction
Heterodimer with other IKAROS family members.
Cell Targets
Component of the chromatin-remodeling NuRD repressor complex which includes at least HDAC1, HDAC2, RBBP4, RBBP7, IKZF1, MTA2, MBD2, MBD3, MTA1L1, CHD3, and CHD4. Interacts directly with the CHD4 component of the NuRD complex.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. John LB and Ward AC, 2011. Mol. Immunol. 48:1272.
2. Merkenschlager M, 2010. FEBS Lett. 584:4910.
3. Thomas RM, et al. 2010. J. Biol. Chem. 285:2545.
4. Papathanasiou P, et al. 2009. Stem Cell. 27:3082.
5. Molnar A, et al. 1996. J. Immunol. 156:585.

Gene ID
22778 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.16.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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