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Purified anti-mouse Ikaros Antibody

Pricing & Availability
Clone
2A9/Ikaros (See other available formats)
Regulatory Status
RUO
Other Names
IKAROS family zinc finger 1, DNA-binding protein Ikaros
Isotype
Mouse IgG1, κ
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Product Citations
publications
2A9slashIkaros_PURE_Ikaros_Antibody_WB_101716
Total cell lysate from Daudi (lane 1, 15 µg), Jurkat (lane 2, 15 µg), A20 (lane 3, 15 µg) and mouse thymus (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse Ikaros antibody (clone 2A9/Ikaros). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 2A9slashIkaros_PURE_Ikaros_Antibody_WB_101716
    Total cell lysate from Daudi (lane 1, 15 µg), Jurkat (lane 2, 15 µg), A20 (lane 3, 15 µg) and mouse thymus (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse Ikaros antibody (clone 2A9/Ikaros). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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653301 25 µg 141 CHF
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653302 100 µg 347 CHF
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Description

Ikaros family members, including Ikaros and Helios, play a role in the development of lymphocytes and B and T cells. They are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and IL-2 mediated signaling events.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal half of mouse Ikaros fused to GST.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported references (for the relevant formats) include: immunofluorescence1 and immunoprecipitation2.

Does not cross-react with human.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

View more applications data for a different format of this clone in our Scientific Poster Library.

This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes.  Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited.  In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Cobb SB, et al. 2000. Genes & Dev. 14:2146. (IF)
  2. Hahm K. et al. 1998. Genes Dev. 12:782. (IP)
  3. Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
Product Citations
  1. Gavish A, et al. 2023. iScience. 26:105826. PubMed
  2. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
RRID
AB_2561711 (BioLegend Cat. No. 653301)
AB_2561711 (BioLegend Cat. No. 653302)

Antigen Details

Structure
Belongs to the Ikaros C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers. 519 amino acids with predicted MW of 58 kD.
Distribution

Nucleus. Abundantly expressed in thymus, spleen and peripheral blood leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

Function
Transcription regulator of hematopoietic cell differentiation. Plays a role in the development of lymphocytes, B and T cells.
Interaction
Heterodimer with other IKAROS family members.
Cell Targets
Component of the chromatin-remodeling NuRD repressor complex which includes at least HDAC1, HDAC2, RBBP4, RBBP7, IKZF1, MTA2, MBD2, MBD3, MTA1L1, CHD3, and CHD4. Interacts directly with the CHD4 component of the NuRD complex.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. John LB and Ward AC, 2011. Mol. Immunol. 48:1272.
2. Merkenschlager M, 2010. FEBS Lett. 584:4910.
3. Thomas RM, et al. 2010. J. Biol. Chem. 285:2545.
4. Papathanasiou P, et al. 2009. Stem Cell. 27:3082.
5. Molnar A, et al. 1996. J. Immunol. 156:585.

Gene ID
22778 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org
Go To Top Version: 6    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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