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Alexa Fluor® 647 anti-mouse Ly49D Antibody

Pricing & Availability
Clone
4E5 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-49D
Isotype
Rat IgG2a, κ
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Product Citations
publications
4E5_AF647_1_051710
C57BL/6 splenocytes stained with CD49b (DX5) PE and 4E5 Alexa Fluor® 647
  • 4E5_AF647_1_051710
    C57BL/6 splenocytes stained with CD49b (DX5) PE and 4E5 Alexa Fluor® 647
  • 4E5_AF647_2_051710
    C57BL/6 splenocytes stained with CD49b (DX5) PE and rat IgG2a Alexa Fluor® 647
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138306 100 µg 288 CHF
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Description

Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.  Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J.  The receptors form homodimers that bind to MHC class I alloantigens.  Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif.  The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens.  Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells.  In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
  2. Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
  3. George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
  4. Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
  5. Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)
Product Citations
  1. Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed
  2. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
RRID
AB_10574955 (BioLegend Cat. No. 138306)

Antigen Details

Structure
A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution

Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.

Function
Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand/Receptor
MHC class I alloantigens
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.

Gene ID
16635 View all products for this Gene ID
UniProt
View information about Ly49D on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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