Alexa Fluor 647 anti-Nestin Antibody anti-Nestin

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Clone: Rat-401 (See other available formats)
Regulatory Status: RUO
Other Names: Nestin, NES
Isotype: Mouse IgG1, κ
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Rat-401_A647_Nestin_Antibody_IHC-P_032818 — IHC staining of Alexa Fluor® 647 anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded Embryonic Day 15 mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody 2 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm

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655107

25 µg

159 CHF

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655108

100 µg

358 CHF

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Description

Nestin was originally identified as a neuronal stem cell marker during central nervous system (CNS) development. It belongs to an intermediate filament protein family, and it is the most widely used marker for identifying neuronal stem cells. Nestin is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. However, the exact function of nestin is not very clear. Nestin is involved in structural organization of the cells through assembling into heterodimers with vimentin or α-internexin in a phosphorylation-dependent process to form intermediate filaments. The expression of nestin is downregulated and replaced by expression of the neurofilament proteins upon neural differentiation/maturation.

Expression of nestin has also been reported in non-neuronal immature or progenitor cells such as pancreatic islet progenitor cells, angiogenic endothelial cells, glioma cells, and bone marrow mesenchymal stem cells. Recent studies also showed that nestin expression can be detected in repair processes in the CNS and in various tumor cells. Thus, it has been used as a cancer stem cell marker. The usage of nestin as a novel prognostic indicator and possible target for tumor anigogenesis has been also proposed.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Homogenized Sprague-Dawley rat spinal cord.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue specificity of this Alexa Fluor® 647 anti-Nestin antibody was validated in embryonic day 15 mouse brain tissue.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: The reactivity of Alexa Fluor® 647 anti-Nestin antibody was verified in FFPE embryonic mouse tissue.

Additional reported applications (for the relevant formats) include: immunofluorescence, immunohistochemistry, and immunoprecipitation^1-4.
RRID: AB_2728489 (BioLegend Cat. No. 655107)
AB_2728489 (BioLegend Cat. No. 655108)

Antigen Details

Structure: Mouse nestin is 1864 amino acids with a predicted molecular weight of 207 kD. The observed molecular weight is larger than 250 kD.
Distribution: Cytosol
Function: Nestin gene encodes a member of the intermediate filament, and it is required for survival and renewal of neural progenitor cells.
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References: 1. Lendahl U, et al. 1990. Cell 60:585.
2. Gilyarov AV, et al. 2008. Neurosci. Behav. Physiol. 38:165.
3. Méndez-Ferrer S, et al. 2010. Nature 466:829.
4. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
Gene ID: 10763 View all products for this Gene ID
UniProt: View information about Nestin on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All Nestin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Nestin	Rat-401	IHC-P,IP
Alexa Fluor® 594 anti-Nestin	Rat-401	IHC-P
Alexa Fluor® 647 anti-Nestin	Rat-401	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.29.2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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