Loading...

Brilliant Violet 421™ anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
A_N418_BV421_1_032911
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
  • A_N418_BV421_1_032911
    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
  • B_N418_BV421_2_032911
  • C_N418_IHC_N418_BV421_071112
    C57BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, and stained with CD11c- BV421™(red), B220-Alexa Fluor® 647 (blue), and I-A/I-E-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
117329 125 µL 176 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
117343 50 µg 264 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
117330 500 µL 385 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Liu J, et al. 2019. Immunity. 50:600. PubMed
  2. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  3. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  4. Jiang X, et al. 2023. Cell Discov. 9:7. PubMed
  5. Nettersheim FS, et al. 2023. Front Cardiovasc Med. 9:1076808. PubMed
  6. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  7. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  8. Pfeifhofer-Obermair C, et al. 2022. Bio Protoc. 12:e4378. PubMed
  9. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  10. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  11. Ho HM, et al. 2022. Pharmaceutics. 14:. PubMed
  12. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  13. Kim EH, et al. 2020. Elife. 9:00. PubMed
  14. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  15. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  16. Szulc-D&acedil;browska L, et al. 2017. PLoS One. 12(6):e0179166. PubMed
  17. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  18. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  19. Evans H, et al. 2015. J Immunol. 195: 2973 - 2984. PubMed
  20. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  21. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  22. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  23. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  24. Park S, et al. 2021. Front Immunol. 11:620166. PubMed
  25. Adachi Y, et al. 2022. Nat Commun. 13:5117. PubMed
  26. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  27. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  28. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  29. Saito S, et al. 2020. Nutrients. 12:. PubMed
  30. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  31. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  32. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  33. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  34. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  35. James BR, et al. 2014. Cancer Immunol Immunother. 63:1213-1227. PubMed
  36. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  37. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  38. Gao H, et al. 2021. Cells. 10:. PubMed
  39. Zheng J, et al. 2020. bioRxiv. . PubMed
  40. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  41. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  42. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  43. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  44. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  45. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  46. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  47. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  48. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  49. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  50. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  51. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  52. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  53. Matundan H, et al. 2019. J Virol. 93. PubMed
  54. Matundan HH, et al. 2021. J Virol. 95:e0103621. PubMed
  55. Zheng J, et al. 2020. Nature. . PubMed
  56. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  57. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  58. Jaggi U, et al. 2021. PLoS Pathog. 17:e1009999. PubMed
  59. Giampazolias E, et al. 2021. Cell. . PubMed
  60. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  61. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  62. Xu G, et al. 2022. Front Pharmacol. 13:896601. PubMed
  63. Huang L, et al. 2021. Frontiers in Cellular and Infection Microbiology. 10:606340. PubMed
  64. Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed
  65. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  66. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  67. Serr I, et al. 2016. Proc Natl Acad Sci U S A. 113: E6659 - E6668. PubMed
  68. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  69. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
  70. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  71. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  72. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  73. Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
  74. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  75. Wang Y, et al. 2020. Vaccines (Basel). 8:. PubMed
  76. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  77. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  78. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  79. Cardoso F, et al. 2015. PLoS One. 10: 0131904. PubMed
  80. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_11219593 (BioLegend Cat. No. 117329)
AB_11219593 (BioLegend Cat. No. 117343)
AB_11219593 (BioLegend Cat. No. 117330)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV395™ anti-mouse CD11c N418 FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™) N418 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account