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Alexa Fluor® 647 anti-rat CD43 Antibody

Pricing & Availability
Clone
W3/13 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13
Isotype
Mouse IgG1, κ
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Product Citations
publications
W3slash13_Alx647_021308
LOU rat splenocytes stained with W3/13 Alexa Fluor® 647
  • W3slash13_Alx647_021308
    LOU rat splenocytes stained with W3/13 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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202810 100 µg 265 CHF
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Description

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte membrane glycoproteins
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections1 and paraffin-embedded sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Barclay AN. 1981. Immunology 42:593.
  2. Brown WRA, et al. 1981. Nature 289:456.
  3. Forbes RC, et al. 1983. Transplantation 36:681.
  4. Jung S, et al. 1994. Immunology 83:545.
Product Citations
  1. Abbondanzo S, Chang S 2014. PLoS One. 9:105256. PubMed
  2. Kiefer J, et al. 2021. Front Immunol. 12:641224. PubMed
RRID
AB_2194199 (BioLegend Cat. No. 202810)

Antigen Details

Structure
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
Distribution

Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.

Function
Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor
CD54, Siglec-1
Cell Type
T cells, NK cells, Granulocytes, Monocytes, Thymocytes, Platelets, Plasma cells, B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
  2. Killeen A, et al. 1987. EMBO J. 6(13):4029.
  3. Thurman EC, em>et al. 1998. Intl. Immunol. 10:691.
  4. Onami TM, et al. 2002. J. Immunol. 168:6022.
  5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
  6. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID
20737 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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