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Alexa Fluor® 647 anti-Tubulin-α Antibody

Pricing & Availability
Clone
10D8 (See other available formats)
Regulatory Status
RUO
Other Names
α-tubulin, Tubulin, alpha-ubiquitous chain
Isotype
Mouse IgM, κ
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Product Citations
publications
10D8_A647_Tubulin-alpha_Antibody_031621
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of Tublin-α (clone 10D8) Alexa Fluor® 647 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 10D8_A647_Tubulin-alpha_Antibody_031621
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of Tublin-α (clone 10D8) Alexa Fluor® 647 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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627908 100 µg 347 CHF
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Description

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human α-tubulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
Product Citations
  1. Chatterjee S, et al. 2023. Sci Adv. 9:eade4800. PubMed
  2. Mei Y, et al. 2020. Nat Commun. 2.661111111. PubMed
RRID
AB_2563178 (BioLegend Cat. No. 627908)

Antigen Details

Structure
Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
Distribution

Ubiquitous

Function
Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
Interaction
RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
Modification
Phosphorylation, acetylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.

Gene ID
84790 View all products for this Gene ID
UniProt
View information about Tubulin-alpha on UniProt.org
Go To Top Version: 3    Revision Date: 03.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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