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Alexa Fluor® 647 anti-Ubiquitin Antibody

Pricing & Availability
Clone
P4G7 (See other available formats)
Regulatory Status
RUO
Other Names
Polyubiquitin-C, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, ubiquitin B
Isotype
Mouse IgG1, κ
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Product Citations
publications
P4G7_A647_Ubiquitin_Antibody_1_122619
IHC staining of Alexa Fluor® 647 anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • P4G7_A647_Ubiquitin_Antibody_1_122619
    IHC staining of Alexa Fluor® 647 anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • P4G7_A647_Ubiquitin_Antibody_2_122619
    IHC staining of Alexa Fluor® 647 anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • P4G7_A647_Ubiquitin_Antibody_3_122619
    IHC staining of Alexa Fluor® 647 anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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838709 25 µg 159 CHF
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838710 100 µg 323 CHF
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Description

Ubiquitin is a small (8.5 kD) regulatory protein that is ubiquitously expressed in tissues of eukaryotic organisms. There are four genes in the human genome that produce ubiquitin; UBB, UBC, UBA52 and RPS27A. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB and UBC genes code for polyubiquitin precursor proteins.

Ubiquitination is a post-translational modification where a ubiquitin subunit is attached to a protein. Addition of ubiquitin can signal for degradation via the proteasome, alter cellular location, promote or prevent protein interactions, or affect activity. Ubiquitination is carried out stepwise by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The cascade results in the binding of ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond.

Proteins can be modified either by a single ubiquitin unit (monoubiquitination) or a chain of ubiquitin molecules (polyubiquitination). Proteins destined for degradation by the proteasome are usually ubiquitinated on lysine residues K48 and K29, while other polyubiquitinations (e.g. on K63, K11, K6) and monoubiquitinations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.

A frameshift mutation in ubiquitin B can result in a truncated peptide missing the C-terminal glycine. This abnormal peptide, known as UBB+1, has been shown to accumulate selectively in Alzheimer's disease and other tauopathies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Clone P4G7 was raised against denatured bovine ubiquitin and recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

P4G7 is equivalent to MAb P4D1. The ubiquitin protein is extremely well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Duttler S, et al. 2013. Mol. Cell. 3:379. (WB) PubMed
  2. Wu Y, et al. 2007. Mol. Cell. Biol. 18:6407. (WB) PubMed
RRID
AB_2820139 (BioLegend Cat. No. 838709)
AB_2820139 (BioLegend Cat. No. 838710)

Antigen Details

Structure
Ubiquitin is a 76 amino acid protein with a molecular mass of 8.5 kD.
Distribution

Found in almost all tissues of eukaryotic organisms

Function
Regulatory protein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Autophagosome Markers
Antigen References

1. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
2. Sarkari F, et al. 2013. J. Biol. Chem. 23:16975. (WB) PubMed

Gene ID
7316 View all products for this Gene ID
UniProt
View information about Ubiquitin on UniProt.org
Go To Top Version: 1    Revision Date: 12.26.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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