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Alexa Fluor® 700 anti-human Ig light chain κ Antibody

Pricing & Availability
Clone
MHK-49 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin light chain kappa
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHK49_AF700_Ig_light_chain_kappa_Antibody_FC_1_021113
Human peripheral blood lymphocytes were stained with CD19 FITC and Ig light chain κ (clone MHK-49) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).
  • MHK49_AF700_Ig_light_chain_kappa_Antibody_FC_1_021113
    Human peripheral blood lymphocytes were stained with CD19 FITC and Ig light chain κ (clone MHK-49) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).
  • MHK49_AF700_Ig_light_chain_kappa_Antibody_FC_2_021113
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
316526 100 tests 347 CHF
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Description

The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
RRID
AB_2561821 (BioLegend Cat. No. 316526)

Antigen Details

Structure
Ig family
Distribution

B cells

Cell Type
B cells
Biology Area
Immunology
Gene ID
3514 View all products for this Gene ID
UniProt
View information about Ig light chain kappa on UniProt.org
Go To Top Version: 4    Revision Date: 01.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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