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Alexa Fluor® 700 anti-mouse/rat CD29 Antibody

Pricing & Availability
Clone
HMβ1-1 (See other available formats)
Regulatory Status
RUO
Other Names
integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMbeta1-1_Alx700_021808
C57BL/6 mouse bone marrow cells stained with HMβ1-1 Alexa Fluor® 700
  • HMbeta1-1_Alx700_021808
    C57BL/6 mouse bone marrow cells stained with HMβ1-1 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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102218 100 µg 229 CHF
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Description

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102235 and 102236).

Application References

(PubMed link indicates BioLegend citation)
  1. Noto K, et al. 1995. Int. Immunol. 7:835.
  2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
  3. Jia W, et al. 2005. Blood 106:3854.PubMed
  4. Economopoulou M, et al. 2005. Blood 106:3831.
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
  7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
  8. Kim DT, et al. 2008. Blood 111:2929.PubMed
  9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
  10. Carlson TR, et al. 2008. Development. 135:2193. PubMed
  11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
  12. Baker CM, et al. 2012. PNAS. PubMed.
  13. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
Product Citations
  1. Lloyd-Lewis B, et al. 2022. Cell Rep. 38:110461. PubMed
  2. Varshney R, et al. 2023. iScience. 26:105750. PubMed
  3. Saavedra-Peña RDM, et al. 2022. J Mol Endocrinol. 68:179. PubMed
  4. He T, et al. 2023. iScience. 26:106289. PubMed
  5. Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed
  6. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  7. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  8. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  9. Hsiao WY, et al. 2020. Cell Rep. 33:108223. PubMed
  10. Ayari A, et al. 2020. Commun Biol. 3:237. PubMed
  11. Sebo ZL, et al. 2018. Development. 145. PubMed
  12. Sebo ZL, et al. 2021. Mol Metab. 44:101141. PubMed
  13. Sastre-Perona A, et al. 2019. Cell Stem Cell. 24:390. PubMed
  14. Jilek S, et al. 2013. Mult Scler. . PubMed
RRID
AB_493711 (BioLegend Cat. No. 102218)

Antigen Details

Structure
Integrin family, 130 kD
Distribution

Leukocytes, endothelial cells, smooth muscle, epithelial cells

Function
Adhesion
Ligand/Receptor
Extracellular matrix
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.

Gene ID
16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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