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Anti-GFAP Antibody (Previously Covance catalog# PRB-571C)

Pricing & Availability
Clone
Poly28400 (See other available formats)
Regulatory Status
RUO
Other Names
Glial Fibrillary Acidic Protein
Previously
Covance Catalog# PRB-571C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28400_Purified_GFAP_Antibody_1_121019
Western blot of anti-GFAP antibody (Poly28400). Lane 1, 4: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate; Lane 5: 20 µg of human brain lysate. The blot was incubated with a 1:20000 dilution of the primary antibody overnight at 4°C, followed by incubation with donkey anti-rabbit IgG antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28400_Purified_GFAP_Antibody_1_121019
    Western blot of anti-GFAP antibody (Poly28400). Lane 1, 4: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate; Lane 5: 20 µg of human brain lysate. The blot was incubated with a 1:20000 dilution of the primary antibody overnight at 4°C, followed by incubation with donkey anti-rabbit IgG antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28400_Purified_GFAP_Antibody_2_121019
    IHC staining of anti-GFAP antibody (Poly28400) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:1000 dilution of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 donkey anti-rabbit IgG antibody (Cat. No. 406414) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28400_Purified_GFAP_Antibody_3_121019
    IHC staining of anti-GFAP antibody (Poly28400) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:1000 dilution of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 donkey anti-rabbit IgG antibody (Cat. No. 406414) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28400_Purified_GFAP_Antibody_4_121019
    IHC staining of anti-GFAP antibody (Poly28400) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:1000 dilution of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 donkey anti-rabbit IgG antibody (Cat. No. 406414) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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840001 100 µL 313 CHF
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Description

Glial Fibrillary Acidic Protein (GFAP) was found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kD protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene. All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antiserum was made with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with purified GFAP from bovine spinal cord.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, a dilution range of 1:10000 - 1:20000 is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:1000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.

Additional Product Notes

This product may contain other non-IgG subtypes.

Product Citations
  1. Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed
  2. García Fallit M, et al. 2023. Viruses. 15:. PubMed
  3. Zuliani I, et al. 2020. Neurotherapeutics. . PubMed
  4. Miyamoto Y, et al. 2016. Nat Commun. 7:13478. PubMed
  5. Fischer M, et al. 2021. Alcohol Clin Exp Res. 45:979. PubMed
  6. Zelenova EA, et al. 2021. Cells. 10:. PubMed
RRID
AB_2565444 (BioLegend Cat. No. 840001)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: Cytoskeleton and cytosol

Function
GFAP is a class III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References
  1. van Bodegraven EJ, et al. 2019. Glia. 67:1417-1433.
  2. Pekny M, et al. 2019. Neurosci Lett. 689:45.
  3. Hol EM, et al. 2017. Cold Spring Harb Perspect Biol. 9(12).
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Other Formats

View All GFAP Reagents Request Custom Conjugation
Description Clone Applications
Anti-GFAP Poly28400 WB,IHC-P
Go To Top Version: 4    Revision Date: 04.27.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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