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APC/Cyanine7 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
  • 30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
    C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103115 25 µg 98 CHF
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103116 100 µg 196 CHF
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Contijoch EJ et al. 2019. eLife. 8 pii: e40553. PubMed
  3. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  4. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  5. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  6. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  7. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  8. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  9. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  10. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  11. Stefkovich M, et al. 2021. Mol Metab. 54:101357. PubMed
  12. Wang Q, et al. 2020. Radiat Environ Biophys. 59:89. PubMed
  13. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  14. De Vries LCS, et al. 2021. J Crohns Colitis. 15:617. PubMed
  15. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  16. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  17. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  18. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  19. Ren D, et al. 2022. STAR Protoc. 3:101217. PubMed
  20. Ren Y, et al. 2021. Am J Transplant. 21:552. PubMed
  21. Hilfenhaus G, et al. 2021. Cancer Res. 81:619. PubMed
  22. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  23. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  24. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  25. He D, et al. 2022. Immunity. 55:159. PubMed
  26. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  27. Goncharov NV, et al. 2022. J Evol Biochem Physiol. 58:230. PubMed
  28. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  29. Denorme F, et al. 2022. J Clin Invest. 132: . PubMed
  30. Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed
  31. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  32. Miyao T, et al. 2022. Elife. 11: . PubMed
  33. Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed
  34. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  35. Campisi L, et al. 2022. Nature. 606:945. PubMed
  36. Kenigsbuch M, et al. 2022. Nat Neurosci. 25:876. PubMed
  37. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  38. Li F, et al. 2022. Gut Microbes. 14:2096989. PubMed
  39. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  40. Zhu J, et al. 2022. Research (Wash D C). 2022:9816272. PubMed
  41. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  42. Zhu X, et al. 2023. Adv Mater. 35:e2207198. PubMed
  43. Li S, et al. 2022. Nat Commun. 13:7371. PubMed
  44. Morrow RJ, et al. 2022. Cells. 11: . PubMed
  45. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  46. Alula KM, et al. 2023. Sci Rep. 13:18. PubMed
  47. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  48. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  49. Zhou J, et al. 2023. Nutrients. 15: . PubMed
  50. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  51. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  52. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
  53. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  54. Chun J, et al. 2023. Cancers (Basel). 15: . PubMed
  55. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  56. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  57. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
  58. Marsh SE, et al. 2022. Nat Neurosci. 25:306. PubMed
  59. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  60. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  61. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  62. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
  63. Pattwell SS, et al. 2022. Sci Adv. 8:eabo6789. PubMed
  64. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  65. Ho HM, et al. 2022. Pharmaceutics. 14:. PubMed
  66. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  67. Banerjee K, et al. 2023. Eur J Immunol. 53:e2250161. PubMed
  68. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  69. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  70. Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed
  71. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  72. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  73. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  74. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  75. Freitag PC, et al. 2023. Mol Ther Methods Clin Dev. 29:120. PubMed
  76. Thai LM, et al. 2023. iScience. 26:106477. PubMed
  77. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  78. Sasaki L, et al. 2022. Nat Aging. 2:105. PubMed
  79. Haensel D, et al. 2023. Nat Commun. 14:2685. PubMed
  80. Lee K, et al. 2023. JCI Insight. 8:. PubMed
  81. Wang C, et al. 2023. Int J Oral Sci. 15:19. PubMed
  82. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  83. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  84. Yang QC, et al. 2023. iScience. 26:106916. PubMed
  85. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  86. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  87. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  88. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  89. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  90. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  91. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  92. Urban SK, et al. 2020. Liver Int. 40:3103. PubMed
  93. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  94. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  95. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  96. Page N, et al. 2018. Immunity. 48:937. PubMed
  97. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  98. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  99. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  100. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  101. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  102. Ebneter A, et al. 2017. Transl Vis Sci Technol. 10.1167/tvst.6.2.10. PubMed
  103. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  104. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  105. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  106. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  107. Pluchino N, et al. 2020. J Cell Mol Med. 24:2464. PubMed
  108. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  109. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  110. Evans H, et al. 2015. J Immunol. 195: 2973 - 2984. PubMed
  111. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  112. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  113. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  114. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  115. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  116. Baram T, et al. 2021. Cells. 10:. PubMed
  117. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  118. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  119. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  120. Adrover JM, et al. 2022. JCI Insight. 7:. PubMed
  121. Steubing RD, et al. 2022. Brain Behav Immun Health. 24:100493. PubMed
  122. Lu H, et al. 2021. Autophagy. 17:2511. PubMed
  123. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
  124. Zhu S, et al. 2022. Front Pharmacol. 13:996635. PubMed
  125. Palacios LM, et al. 2022. Biology (Basel). 11:. PubMed
  126. Zhou X, et al. 2022. Front Pharmacol. 13:887263. PubMed
  127. Biswas S, et al. 2021. Nature. 591:464. PubMed
  128. Forte E, et al. 2020. J Cell Mol Med. . PubMed
  129. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  130. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  131. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  132. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  133. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  134. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  135. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  136. Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed
  137. Wiley CD, et al. 2019. JCI Insight. 4:e130056. PubMed
  138. Krasteva G, et al. 2011. Proc Natl Acad Sci U S A. 108:9478. PubMed
  139. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  140. Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
  141. Mantia-Smaldone G, et al. 2014. Gynecol Oncol. 133:584. PubMed
  142. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  143. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  144. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  145. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  146. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  147. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  148. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  149. Shi S, et al. 2020. Clin Cancer Res. 26:5990. PubMed
  150. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  151. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  152. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  153. Jung BG, et al. 2022. PLoS Pathog. 18:e1010454. PubMed
  154. Schönfeld M, et al. 2021. Alcohol Clin Exp Res. 45:1980. PubMed
  155. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  156. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  157. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  158. Marangoni F, et al. 2021. Cell. . PubMed
  159. Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed
  160. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  161. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  162. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  163. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  164. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  165. Ning F, et al. 2019. J Exp Med. 216:1396. PubMed
  166. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  167. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  168. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  169. Hammond TR, et al. 2019. Immunity. 50:253. PubMed
  170. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  171. Commerford CD et al. 2018. Cell reports. . PubMed
  172. Zeng J, et al. 2019. Cell Rep. 27:549. PubMed
  173. Yousef H, et al. 2018. Bio Protoc. 8:22. PubMed
  174. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  175. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  176. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  177. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  178. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  179. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  180. Liu M, et al. 2020. Cell Rep. 32:107990. PubMed
  181. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  182. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  183. Chi Z, et al. 2020. Molecular Cell. 80(1):43-58.e7. PubMed
  184. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  185. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  186. Uher O, et al. 2021. Cancers (Basel). 13:. PubMed
  187. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  188. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  189. Gao H, et al. 2021. Cells. 10:. PubMed
  190. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  191. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  192. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  193. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  194. Zhang X, et al. 2020. Elife. 9:00. PubMed
  195. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  196. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  197. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  198. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  199. Soldevilla MM, et al. 2019. Mol Ther. 27:1878. PubMed
  200. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  201. Li Y, et al. 2019. Sci Rep. 9:6932. PubMed
  202. Ayaub E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13511-z. PubMed
  203. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  204. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  205. Veremeyko T, et al. 2012. J Vis Exp. 65: 4097. PubMed
  206. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  207. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  208. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  209. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  210. Ali M, et al. 2022. JCI Insight. 7:. PubMed
  211. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  212. Cho YK, et al. 2022. Nat Commun. 13:4084. PubMed
  213. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
  214. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  215. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  216. Neuhöfer P, et al. 2021. Nature. 597:715. PubMed
  217. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  218. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  219. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  220. Selvaraj UM, et al. 2021. Brain Behav Immun. 95:502. PubMed
  221. Ying T, et al. 2021. Am J Physiol Endocrinol Metab. 320:E1148. PubMed
  222. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  223. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  224. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  225. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  226. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  227. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  228. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  229. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  230. Martina M, et al. 2014. J Vis Exp. 87: 51192. PubMed
  231. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  232. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  233. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  234. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  235. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  236. Abramowski P, et al. 2014. J Neuroimmunol. 274:111. PubMed
  237. Hoeksema M, et al. 2015. J Immunol. 194:3909. PubMed
  238. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  239. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  240. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  241. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  242. Yang Y, et al. 2022. Elife. 11:. PubMed
  243. Wang R, et al. 2022. Front Immunol. 13:947756. PubMed
  244. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  245. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  246. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  247. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  248. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  249. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  250. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  251. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  252. Gierut J, et al. 2015. Stem Cell Res. 15: 165-171. PubMed
  253. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  254. Haka A, et al. 2012. PLoS One. 7:e44823. PubMed
  255. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  256. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  257. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  258. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  259. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  260. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  261. Camara A, et al. 2019. Immunity. 50:1467. PubMed
  262. Michaelis KA, et al. 2019. Brain Behav Immun. 82:338. PubMed
  263. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  264. Favuzzi E, et al. 2021. Cell. 184(15):4048-4063.e32. PubMed
  265. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  266. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  267. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  268. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  269. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  270. Yu W, et al. 2022. Nat Commun. 13:3544. PubMed
  271. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  272. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  273. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  274. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  275. Wang X, et al. 2022. Front Immunol. 13:864984. PubMed
  276. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  277. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  278. Ou YC, et al. 2020. ACS Nano. 1.035416667. PubMed
  279. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  280. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  281. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  282. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  283. Stock M, et al. 2015. Osteoarthritis Cartilage. 23: 1221-1225. PubMed
  284. Draper D, et al. 2012. J Immunol. 188:5327. PubMed
  285. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  286. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  287. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  288. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  289. Selvaraj UM et al. 2018. Endocrinology. 159(11):3848-3859 . PubMed
  290. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  291. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  292. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  293. Kaiser T, et al. 2019. Eneuro. 6. PubMed
  294. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  295. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  296. Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed
  297. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  298. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  299. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  300. Lu Z, et al. 2021. J Clin Invest. 131:. PubMed
  301. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  302. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  303. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  304. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  305. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  306. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  307. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  308. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
  309. Kline JM, et al. 2021. J Biol Chem. :100373. PubMed
  310. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  311. Starikova E, et al. 2021. Front Pharmacol. 12:667572. PubMed
  312. Klein S, et al. 2016. J Cell Sci. 129: 2573 - 2585. PubMed
  313. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  314. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  315. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  316. Jiang P, et al. 2018. Nat Med. 24:1550. PubMed
  317. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  318. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  319. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  320. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  321. Martin A, et al. 2018. Oncotarget. 9:28364. PubMed
  322. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  323. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  324. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  325. Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed
  326. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  327. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  328. Yoldi G, et al. 2016. Cancer Res . 76: 5857 - 5869. PubMed
  329. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  330. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  331. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  332. Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
  333. Morein D, et al. 2021. Cells. 10: . PubMed
  334. Yan C, et al. 2022. NPJ Precis Oncol. 6:6. PubMed
  335. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  336. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  337. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  338. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  339. Wang Y, et al. 2022. Nat Commun. 13:1454. PubMed
  340. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  341. Zhang X, et al. 2022. J Exp Clin Cancer Res. 41:246. PubMed
  342. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  343. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  344. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  345. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  346. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  347. Liu SS, et al. 2021. Immunity. :. PubMed
  348. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  349. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  350. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  351. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  352. Seeling M, et al. 2013. Proc Natl Acad Sci U S A. 110:10729. PubMed
  353. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  354. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  355. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  356. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  357. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  358. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  359. Forte E, et al. 2020. Cell Rep. 30:3149. PubMed
  360. Wang J, et al. 2020. J Hematol Oncol. 0.610416667. PubMed
  361. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  362. Lux A, et al. 2014. Cell Rep. 10:236. PubMed
  363. Madsen P, et al. 2016. J Neurosci. 36: 5128 - 5143. PubMed
  364. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  365. Parker K, Horn L, Ostrand-Rosenberg S 2016. J Leukoc Biol. 100: 463 - 470.. PubMed
  366. Chen MB, et al. 2020. Cell Rep. 30:4418. PubMed
  367. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  368. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  369. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  370. De Micheli AJ, et al. 2020. Am J Physiol Cell Physiol. 319:C885. PubMed
  371. Sibler E, et al. 2021. Cells. 10:. PubMed
  372. Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed
  373. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  374. Sebastian A, et al. 2021. Cells. 10:. PubMed
  375. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  376. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  377. Swanson WB, et al. 2021. Biomaterials. 272:120769. PubMed
  378. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  379. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  380. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  381. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  382. Parent AV, et al. 2021. Cell Reports. 36(7):109538. PubMed
  383. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  384. Ma Y, et al. 2021. Developmental Cell. 56(4):478-493.e11. PubMed
  385. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  386. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  387. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  388. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  389. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
RRID
AB_312980 (BioLegend Cat. No. 103115)
AB_312980 (BioLegend Cat. No. 103116)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 3    Revision Date: 01.30.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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