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Anti-Involucrin Antibody (Previously Covance catalog# PRB-140C)

Pricing & Availability
Clone
Poly19244 (See other available formats)
Regulatory Status
RUO
Other Names
IVL
Previously
Covance Catalog# PRB-140C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
a-Poly19244_Purified_Involucrin_2_011918
IHC staining of anti-Involucrin antibody (clone Poly19244) on formalin-fixed paraffin-embedded mouse skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:1000 dilution of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • a-Poly19244_Purified_Involucrin_2_011918
    IHC staining of anti-Involucrin antibody (clone Poly19244) on formalin-fixed paraffin-embedded mouse skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:1000 dilution of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • b-Poly19244_Purified_Involucrin_1_011918
    Total lysates (15 µg protein) from HaCaT (Positive control) and THP-1 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:5000 Involucrin antibody, clone Poly19244. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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924401 200 µL 375 CHF
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Description

Involucrin serves as a marker for terminal differentiation of stratified squamous epithelia. The involucrin genes have diverged considerably through evolution and as a consequence antibodies specific for the human molecule do not detect the homologous mouse gene product.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
This polyclonal antibody was generated against an 18 amino acid peptide (ELHLGKQQESHEPDMAED) predicted from the mouse involucrin gene sequence.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IHC-P - Quality tested
WB - Verified
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:500 - 1:1000 is suggested. For Western blotting, the suggested use of this reagent is 1:1000 - 1:5000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Wallace L, et al. 2012. J. Cell Sci. 125: 1750. (IHC-F, WB) PubMed
  2. Jennemann R, et al. 2012. 21: 586. (IHC-P, WB)
  3. Merritt AJ, et al. 2002. Mol Cell Biol. 22:5846. (IHC-P, WB)
  4. Lin AW, Lowe SW. 2001. PNAS. 98:5025. (IHC-F, WB, ICC)
  5. Djian P, et al. 2000. J Cell Biol. 151:F5. (ICC, WB, IHC-P) PubMed
  6. Li ER, et al. 2000. Exp Dermatol. 9:431. (WB)
  7. Bayo P, et al. 2008. Endocrinology. 149: 1377. (IHC-P) PubMed
Product Citations
  1. Sylakowski K, et al. 2022. Wound Repair Regen. 30:436. PubMed
  2. Kantzer CG, et al. 2022. Development. 149:. PubMed
  3. Butera A, et al. 2023. Sci Adv. 9:eadg5423. PubMed
  4. Pal-Ghosh S, et al. 2020. Invest Ophthalmol Vis Sci. 61:01:00. PubMed
  5. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  6. Xi L, et al. 2020. Elife. 9:00. PubMed
  7. Hasche D, et al. 2016. PLoS One. 11: 0161283. PubMed
  8. Bayo P, et al. 2008. Endocrinology. 149:1377-1388. PubMed
  9. Michel C, et al. 2017. J Immunol. 199:3488. PubMed
  10. Naeem A, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31377-X. PubMed
  11. Koenig U, et al. 2019. Autophagy. :1. PubMed
  12. Lopes N, et al. 2022. Elife. 11:. PubMed
  13. Djian P, et al. 2000. J Cell Biol. 151:381-388. PubMed
  14. Carper MB, et al. 2019. Cell Rep. 29:1660. PubMed
  15. Sanz-Gómez N, et al. 2020. Cell Death Differ. 27:2451. PubMed
  16. Wallace L, et al. 2012. J Cell Sci. 125:1750-1758. PubMed
  17. Lecland N, et al. 2019. Life Sci Alliance. 2:2. PubMed
  18. Xiong Z, et al. 2018. J Pathol. 244:164. PubMed
  19. Buerger C, et al. 2017. PLoS One. 10.1371/journal.pone.0180853. PubMed
RRID
AB_2565452 (BioLegend Cat. No. 924401)

Antigen Details

Structure
Predicted MW = ~68 kD, however bands ~78 kD are detectable depending on nature of source material (refer to Li ER et al paper below).
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
16447 View all products for this Gene ID
UniProt
View information about Involucrin on UniProt.org

Other Formats

View All Involucrin Reagents Request Custom Conjugation
Description Clone Applications
Anti-Involucrin Poly19244 IHC-P,WB,IHC-F,ICC
Go To Top Version: 4    Revision Date: 04.27.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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