- Clone
- Poly19056 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Keratin, type II cytoskeletal 1, K1, CK-1, keratin 86, cytokeratin-1, 67 kD cytokeratin, type-II keratin Kb1, keratin complex 2, basic, gene 1
- Previously
-
Covance Catalog# PRB-165P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
905602 | 25 µL | 89 CHF | ||||
905601 | 100 µL | 288 CHF |
Keratin 1 belongs to the keratin family of proteins which are fibrous structural proteins. It is a type II cytokeratin that forms the major cytoskeleton in suprabasal keratinocytes along with heterodimer type I partner KRT10 form. KRT1 and KRT10 mutations lead to congenital epidermolytic ichthyosis characterized by skin erosions and hyperkeratosis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 1 protein.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
WB - Verified
IHC-F - Reported in the literature, not tested in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution of 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1.0 µg per mL (1:1000 dilution). For immunocytochemistry, a dilution of 1:500 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been successfully used in both frozen and paraffin embedded tissues.
*Predicted MW = 67 kD
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Croyle MJ, et al. 2011. Development 138: 1675. (IHC, IF) PubMed
- Wirt SE, et al. 2010. J. Cell Biol. 191: 809. (IHC) PubMed
- Hu Y, et al. 2001. Nature. 410:710.
- Yuspa SH, et al. 1989. J Cell Biol. 109:1207.
- Roop DR, et al. 1984. J Biol Chem. 259:8037.
- Mailleux AA, et al. 2007. Dec Cell. 12:221. (IHC)
- IShitsuka Y, et al. 2013. J Invest Dermatol. 133:2566. (WB)
- Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
- Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
- Levy V, et al. 2007. FASEB J. 21:1358. (IHC) PubMed
- Osakarsson T, et al. 2006. Genes Dev. 20:2024. (IHC) PubMed
- Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
- Sachs N, et al. 2012. PNAS. 109:21468. (IF)
- Vidal VP, et al. 2005. Curr Biol. 15:1340. (IHC)
- Product Citations
-
- RRID
-
AB_2801250 (BioLegend Cat. No. 905602)
AB_2801250 (BioLegend Cat. No. 905601)
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 16678 View all products for this Gene ID
- UniProt
- View information about Keratin 1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Keratin 1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Keratin 1 | Poly19056 | IHC-P,WB,IHC-F |
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