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Anti-Prox1 Antibody (Previously Covance catalog# PRB-238C)

Pricing & Availability
Clone
Poly19252 (See other available formats)
Regulatory Status
RUO
Other Names
Prospero Homeobox Protein 1, Prox-1, Prospero-Related Homeobox 1, Homeobox Prospero-Like Protein Prox1
Previously
Covance Catalog# PRB-238C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly19252_Anti-Prox1_Serum_IF_1_030315.jpg
Prox1 (Poly19252) detection on mouse brain. Photo courtesy of C. Zhou & S. Pleasure, UCSF.
  • Poly19252_Anti-Prox1_Serum_IF_1_030315.jpg
    Prox1 (Poly19252) detection on mouse brain. Photo courtesy of C. Zhou & S. Pleasure, UCSF.
  • Poly19252_Anti-Prox1_Serum_IF_2_030315.jpg
    Prox1 (Poly19252) detection on mouse brain. Photo courtesy of S. Pleasure, UCSF.
  • Poly19252_Anti-Prox1_Serum_WB_3_030315.jpg
    Total lysates (15 µg protein) from HepG2 (Positive control) and HL-60 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 purified Prox1 antibody, clone Poly19052. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # Size Price Quantity Check Availability Save
925202 25 µL 89 CHF
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925201 200 µL 352 CHF
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Description

Prox1 is a prospero related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues. Prox1-deficient mice are devoid of lymphatic vasculature, and in these animals endothelial cells fail to acquire the lymphatic phenotype; instead, they remain as blood vascular endothelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The Prox1 antiserum was generated against the C-terminal 15 amino acids of mouse Prox1.
Formulation
Stored in 50% Glycerol and 0.03% Thimerosal.
Preparation
Serum
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution of 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:500 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Expected MW: 83 kD

WB Positive Control: HEK293 Lysate

IHC Positive Control: Mouse Brain


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Pierreux CE, et al. 2004. J. Biol. Chem. 279:51298.
  2. Bagri A, et al. 2003. Cell. 113:285.
  3. Bagri A, et al. 2002. Development. 129:4249.
Product Citations
  1. Filin IY, et al. 2023. Curr Issues Mol Biol. 7827:45. PubMed
  2. Nieuwenhuis B, et al. 2023. Gene Ther. :. PubMed
  3. Macova I, et al. 2019. J Neurosci. 39:984. PubMed
  4. Karnezis T et al. 2019. Cancer Res. 79(7):1558-1572 . PubMed
  5. Keeley PW, et al. 2018. Vis Neurosci. 35:E003. PubMed
  6. Banerjee S, et al. 2021. Visual Neuroscience. 38:E008. PubMed
  7. Filova I, et al. 2020. Mol Neurobiol. 57:5307. PubMed
  8. Nguyen PT, et al. 2020. Cell. 182(2):388-403.e15. PubMed
  9. Keeley PW, et al. 2018. J Comp Neurol. 526:467. PubMed
  10. Castillo S, et al. 2016. Sci Transl Med. 8: 332ra43. PubMed
  11. Luna E, et al. 2018. Acta Neuropathol. 135:855. PubMed
  12. Buckmaster PS, et al. 2017. J Comp Neurol. 525:2592. PubMed
  13. Prajapati–DiNubila M, et al. 2019. Elife. 8. PubMed
  14. Ning K, et al. 2021. Invest Ophthalmol Vis Sci. 62:15. PubMed
RRID
AB_2749865 (BioLegend Cat. No. 925202)
AB_2749865 (BioLegend Cat. No. 925201)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
Gene ID
5629 View all products for this Gene ID
UniProt
View information about Prox1 on UniProt.org

Other Formats

View All Prox1 Reagents Request Custom Conjugation
Description Clone Applications
Anti-Prox1 Poly19252 IHC-F,WB,ICC
Go To Top Version: 2    Revision Date: 07.26.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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