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Purified Non-muscle Myosin Heavy Chain II-B Antibody (Previously Covance catalog# PRB-445P)

Pricing & Availability
Clone
Poly19099 (See other available formats)
Regulatory Status
RUO
Other Names
Myosin-10, NMMHC-B, myosin heavy chain 10, nonmuscle myosin heavy chain-B, nonmuscle myosin heavy chain IIB, cellular myosin heavy chain, type B type B
Previously
Covance Catalog# PRB-445P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly19099_Pure_ChainII-B_Antibody_1_111417
Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • Poly19099_Pure_ChainII-B_Antibody_1_111417
    Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • Poly19099_Pure_ChainII-B_Antibody_2_111417
    Immunofluorescence of HeLa cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Non-muscle Myosin Heavy Chain II-B primary Antibody. Alexa Fluor® 594 (Red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/60. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml, (D) is 0.5 µg/ml.
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909902 25 µL 89 CHF
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909901 100 µL 288 CHF
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Description

Non-muscle Myosin Heavy Chain II-B is a member of the myosin superfamily. There are three isoforms of myosin II, called myosin IIA, myosin IIB and myosin IIC. These proteins are involved in cell motility, maintenance of cell shape, and cytokinesis. Each type of myosin II protein consists of two heavy chains and four light chains. The heavy chains each have two parts: a myosin head-like domain and a tail region. The head region interacts with actin, a protein that is important for cell movement and shape. The long tail region interacts with other proteins, including the tail regions of other myosin proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B).
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:1000 - 1:2000). For immunocytochemistry, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF).

*Predicted MW = 200 kD

Positive Control: human smooth muscle


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma X, et al. 2007. Mol Biol Cell. 18:2305. (IF)
  2. Golomb E, et al. 2004. J Biol Chem. 279:2800. (WB, IF)
  3. Song J, et al. 2001. Mol Cell Neurosci. 17:624.
  4. Kelley CA, et al. 1996. J Cell Biol. 134:675.
  5. Kelley CA, et al. 1995. J Biol Chem. 270:1395.
  6. Phillips CL, et al. 1995. J Muscl Res Cell Motil. 16:379.
  7. Skau CT, et al. 2015. PNAS. 112:2447. PubMed
  8. Wang H, et al. 2008. J. Cell Biol. 182: 1171. (ICC, WB) PubMed
  9. Hirota Y, et al. 2010. Development. 137: 3037. (IF) PubMed
Product Citations
  1. Chopra A, et al. 2018. Dev Cell. 44:87. PubMed
  2. Wang XW, et al. 2020. Cell Rep. 31:107537. PubMed
  3. Fujiwara S, et al. 2022. Ann N Y Acad Sci. 1516:85. PubMed
  4. D'Arcy BR, et al. 2023. PLoS Biol. 21:e3001926. PubMed
  5. Francou A, et al. 2023. Elife. 12:. PubMed
  6. Rouaud F, et al. 2023. J Cell Biol. 222:. PubMed
  7. Weißenbruch K, et al. 2022. Eur J Cell Biol. 101:151213. PubMed
  8. Tokuda S, Hirai T, and Furuse M. 2017. PLoS One.. 10.1371/journal.pone.0182521. PubMed
  9. Priya R, et al. 2017. Mol Biol Cell. 28(1):12-20. PubMed
  10. Cartagena-Rivera A, Van Itallie C, and Anderson J. 2017. Nat Commun. . 10.1038/s41467-017-01145-8. PubMed
  11. Li X, et al. 2015. Proc Natl Acad Sci U S A. 112:6080. PubMed
  12. Aguilar-Cuenca R, et al. 2020. Current Biology. 30(13):2446-2458.e6. PubMed
  13. Belardi B, et al. 2020. Developmental Cell. 54(6):792-804.e7. PubMed
  14. Bonneaud TL, et al. 2022. Cell Death Dis. 13:787. PubMed
  15. Otterpohl K, et al. 2017. Physiol Rep.. 10.14814/phy2.13513. PubMed
  16. Sumigray KD et al. 2018. Developmental cell. 45(2):183-197 . PubMed
  17. Martínez-Ara G, et al. 2022. Nat Commun. 13:5400. PubMed
  18. Jiu Y, et al. 2019. Curr Biol. 29:81. PubMed
  19. Eaton AF, et al. 2019. Mol Biol Cell. 30:2037. PubMed
  20. S Tokuda, T Hirai, M Furuse 2016. PLoS One. 11:e0166904. PubMed
  21. Yu-Kemp HC, et al. 2022. J Cell Biol. 221:. PubMed
  22. Weienbruch K, et al. 2021. eLife. 0.416666666666667. PubMed
  23. Salomon J, et al. 2017. Nat Commun. 8:13998. PubMed
  24. Hirota Y, et al. 2010. Development. 137:3037-3046. PubMed
  25. Ninomiya K, et al. 2021. J Cell Sci. 134:. PubMed
  26. Zhou R, et al. 2022. Nat Commun. 13:3196. PubMed
  27. Butler MB, et al. 2019. J Cell Sci. 132. PubMed
  28. Ninomiya K, et al. 2020. J Cell Sci. . PubMed
  29. Zhovmer AS, et al. 2019. Mol Biol Cell. 30:1961. PubMed
  30. Naydenov NG, et al. 2022. Cells. 11:. PubMed
  31. Skau C, et al. 2015. Proc Natl Acad Sci U S A. 112: 2447 - 2456. PubMed
  32. Trivedi N, et al. 2017. Nat Commun. 8:14484. PubMed
  33. Ramkumar N, et al. 2016. Nat Cell Biol. 18:1281-1291. PubMed
  34. Awadia S, et al. 2019. J Cell Biol. 218:2699. PubMed
  35. Zaman R, et al. 2021. J Cell Biol. 220:. PubMed
  36. Van Itallie CM, et al. 2018. J Cell Sci. 131:. PubMed
  37. Wang H, et al. 2008. J Cell Biol. 182:1171-1184. PubMed
  38. Maniou E, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  39. Loebel D, et al. 2016. Biol Open. 10.1242/bio.014415. PubMed
  40. Yamada S, et al. 2019. J Dent Res. 98:1253. PubMed
  41. Fenix AM, et al. 2018. Elife. 7:. PubMed
  42. Nguyen-Ngoc K, et al. 2017. J Cell Sci. 10.1242/jcs.208546. PubMed
  43. Ning W, et al. 2020. Cell Stem Cell. 28(3):436-452.e5. PubMed
RRID
AB_2565101 (BioLegend Cat. No. 909902)
AB_2565101 (BioLegend Cat. No. 909901)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
4628 View all products for this Gene ID
UniProt
View information about Myosin-10 on UniProt.org
Go To Top Version: 9    Revision Date: 08.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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