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APC anti-mouse CD153 Antibody

Pricing & Availability
Clone
RM153 (See other available formats)
Regulatory Status
RUO
Other Names
CD30 ligand, CD30L, TNFSF8
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RM153_APC_CD153_Antibody_FC_111113
PMA+ionomycin-stimulated (2 day) C57BL/6 mouse splenocytes were stained with CD153 (clone RM153) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • RM153_APC_CD153_Antibody_FC_111113
    PMA+ionomycin-stimulated (2 day) C57BL/6 mouse splenocytes were stained with CD153 (clone RM153) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
See APC spectral data
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106409 50 µg 131 CHF
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Description

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with mouse CD153 gene (Tnfsf8)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30 binding to CD30 ligand (CD153)1-3,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 106408).

Application References

(PubMed link indicates BioLegend citation)
  1. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
  2. Seko Y, et al. 2001. J. Pathol. 195:593.
  3. Akiba H, et al. 2000. J. Exp. Med. 191:375.
  4. Zhang H and GG Meadows 2005. J. Leukocyte Biol. 78:1070.
  5. Zeiser R, et al. 2007. Blood 109:2225.PubMed
  6. Sun X, et al. 2010. J. Immunol. 185:2222. PubMed
RRID
AB_2562680 (BioLegend Cat. No. 106409)

Antigen Details

Structure
TNF superfamily, 39-40 kD
Distribution
Activated T cells
Function
T-B cell costimulation
Ligand/Receptor
CD30
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Horie R, et al. 1998. Semin. Immunol. 106:457.
3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147.
5. Bowen MA, et al. 1996. J. Immunol. 156:442.

Gene ID
21949 View all products for this Gene ID
UniProt
View information about CD153 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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