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APC anti-mouse ESAM Antibody

Pricing & Availability
Clone
1G8/ESAM (See other available formats)
Regulatory Status
RUO
Other Names
Endothelial cell-selective adhesion molecule
Isotype
Rat IgG2a, κ
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Product Citations
publications
1G8ESAM_APC_040610
Mouse endothelial cell line bEnd.3 stained with 1G8/ESAM APC
  • 1G8ESAM_APC_040610
    Mouse endothelial cell line bEnd.3 stained with 1G8/ESAM APC
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136207 25 µg 113 CHF
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Description

Endothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation process. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bEND.3 endothelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Nasdala I, et al. 2002. J. Bio. Chem. 277:16294
Product Citations
  1. Tober J, et al. 2020. The Journal of Experimental Medicine. 215(2):645-659. PubMed
  2. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  3. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  4. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  5. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  6. Yang Z, et al. 2020. J Invest Dermatol. 544:140. PubMed
  7. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
RRID
AB_2101658 (BioLegend Cat. No. 136207)

Antigen Details

Structure
A 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain.
Distribution

Expressed at endothelial junctions, platelets, and murine hematopoietic stem cells.

Function
It impacts vascular permeability and extravasation process. ESAM expression is involved in hematopoietic stem cell (HSC) activity.
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Wegmann F, et al. 2004. Exp Cell Res. 300:121
2. Ooi LAG, et al. 2008. Stem Cells. 27:653
3. Yokota T, et al. 2009. Blood 113(13):2871. Phosphate-buffered solution pH 7.2 containing 0.09% sodium azide.

Gene ID
69524 View all products for this Gene ID
UniProt
View information about ESAM on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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