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APC anti-mouse Granzyme C Antibody

Pricing & Availability
Clone
SFC1D8 (See other available formats)
Regulatory Status
RUO
Other Names
CCP2, Ctla5, Ctla-5, AI323531, B10, GzmC
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
SFC1D8_APC_GranzymeC_Antibody_012119
CD3/CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 PE and Granzyme C (clone SFC1D8) APC (left) or Armenian Hamster IgG APC isotype control (right).
  • SFC1D8_APC_GranzymeC_Antibody_012119
    CD3/CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 PE and Granzyme C (clone SFC1D8) APC (left) or Armenian Hamster IgG APC isotype control (right).
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150811 25 µg 149 CHF
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150812 100 µg 350 CHF
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Description

Granzyme C is a serine protease that is found in the granules of cytotoxic T lymphocytes and natural killer cells (NKs). It induces the apoptosis of target cells by the externalization of phosphatidylserine, nuclear condensation and collapse, as well as the introduction of single-stranded DNA nicks. It can also induce apoptosis via mitochondrial swelling, which results in the loss of membrane potential. Reported studies show that granzyme C can support CTL-mediated killing in the absence of granzyme A or B. Additionally, wild-type mice have delayed upregulation of granzyme C relative to granzyme B and a mutation in the granzyme B gene shows earlier upregulation of granzyme C. Further studies show that in the event that granzyme B fails, the mouse models infected with persistent viruses have delayed expression of protection provided by granzyme C.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified recombinant granzyme C in CFA and boosted in IFA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Cai Sheng, et al. 2009. J. Immunol. 182:6287. (ICFC)
Product Citations
  1. Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed
RRID
AB_2800686 (BioLegend Cat. No. 150811)
AB_2800686 (BioLegend Cat. No. 150812)

Antigen Details

Distribution

Cytotoxic T lymphocytes and natural killer (NK) cells.

Function
Induces apoptotic cell death.
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Bots M, et al. 2006. J. Cell. Sci. 119:5011.
2. Ley TJ, et al. 2003. Blood 101:3093.
3. Revell PA. 2005. J. Immunol. 2005. 174:2124.
4. Kelso A. 2002. Int. Immunol. 6:605.
5. Getachew Y, et al. 2008. J. Immunol. 181:7810.
6. Cai Sheng, et al. 2009. J. Immunol. 182:6287.

Gene ID
14940 View all products for this Gene ID
UniProt
View information about Granzyme C on UniProt.org
Go To Top Version: 1    Revision Date: 01.21.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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