- Clone
- W18174A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sca-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
160903 | 25 µg | 78 CHF | ||||
160904 | 100 µg | 261 CHF |
Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- EL4 Cell
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes.
- RRID
-
AB_2910333 (BioLegend Cat. No. 160903)
AB_2910333 (BioLegend Cat. No. 160904)
Antigen Details
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
-
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Function
- Regulates B and T cell responses
- Cell Type
- B cells, Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells, Thymocytes
- Biology Area
- Stem Cells
- Antigen References
-
- Upadhyay G. 2019. Front Immunol. 10:819.
- Rock KL, et al. 1989. Immunol. Rev. 111:195-224.
- Gene ID
- 110454 View all products for this Gene ID
- UniProt
- View information about Ly-6A/E on UniProt.org
Related FAQs
Other Formats
View All Ly-6A/E Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ly-6A/E (Sca-1) | W18174A | FC |
APC anti-mouse Ly-6A/E (Sca-1) | W18174A | FC |
PE anti-mouse Ly-6A/E (Sca-1) | W18174A | FC |
FITC anti-mouse Ly-6A/E (Sca-1) | W18174A | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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-
Purified anti-mouse Ly-6A/E (Sca-1)
C57BL/6 mouse splenocytes were stained with purified anti-mo... -
APC anti-mouse Ly-6A/E (Sca-1)
C57BL/6 splenocytes were stained with anti-mouse Ly-6A/E (Sc... -
PE anti-mouse Ly-6A/E (Sca-1)
C57BL/6 splenocytes were stained with anti-mouse Ly-6A/E (Sc... -
FITC anti-mouse Ly-6A/E (Sca-1)
C57BL/6 mouse splenocytes were stained with anti-mouse Ly-6A...
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