- Clone
- 34-1-2S (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC Class I
- Isotype
- Mouse (C3H) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A.
34-1-2S_APC-FIRE-750_H2K-H-2D_His-FC_122923 A.
34-1-2S_APC-FIRE-750_H2K-H-2D_His-FC_122923](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/34-1-2S_APC-FIRE-750_H2K-H-2D_His-FC_122923-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse H-2Kd/H-2Dd (clone 34-1-2S) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram). -
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (clone GK1.5) PE and anti-mouse H-2Kd/H-2Dd (clone 34-1-2S) APC/Fire™ 750 (left) or mouse IgG2a, κ APC/Fire™ 750 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
114719 | 25 µg | 106 CHF | ||||
114720 | 100 µg | 270 CHF |
The 34-1-2S antibody reacts with the H-2Kd/H-2Dd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd/H-2Dd haplotype. H-2Kd/H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BDF Splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Ozato K, et al. 1982. Transplantation 34:113.
- Sester M, et al. 2000. J. Biol. Chem. 34:113.
- RRID
-
AB_3097451 (BioLegend Cat. No. 114719)
AB_3097451 (BioLegend Cat. No. 114720)
Antigen Details
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Kd/H-2Dd haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
- Watts C. 1997. Ann. Rev. Immunol. 15:821.
- Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
- York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
- Gene ID
- 14964 View all products for this Gene ID 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kd/H-2Dd on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All H-2Kd/H-2Dd Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
PE anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
Alexa Fluor® 647 anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
APC anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
PerCP/Cyanine5.5 anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
PE/Cyanine7 anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
APC/Fire™ 750 anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
Spark Violet™ 423 anti-mouse H-2Kd/H-2Dd | 34-1-2S | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes stained with 34-1-2S PE C3H/He mouse splenocytes stained with 34-1-2S PE -
PE anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes stained with 34-1-2S PE C57BL/6 mouse splenocytes stained with 34-1-2S PE -
Alexa Fluor® 647 anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes stained with 34-1-2S Alexa Fluor® ... -
APC anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes were stained with mouse H-2Kd... -
PerCP/Cyanine5.5 anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes were stained with mouse H-2Kd... -
PE/Cyanine7 anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes were stained with mouse H-2Kd... -
APC/Fire™ 750 anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes were stained with anti-mouse H-2K<... C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (... -
Spark Violet™ 423 anti-mouse H-2Kd/H-2Dd
C57BL/6 mouse splenocytes were stained with anti-mouse H-2K<... C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (...
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