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Biotin anti-CX3CR1 Antibody

Pricing & Availability
Clone
8E10.D9 (See other available formats)
Regulatory Status
RUO
Other Names
CX3C chemokine receptor 1, fractalkine receptor, beta chemokine receptor-like 1, chemokine (C-C) receptor-like 1, GRP13, V28
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
8E10dotD9_Biotin_CX3CR1_Antibody_IHC-P_010417
IHC staining of anti-CX3CR1 antibody (clone 8E10.D9) conjugated to biotin on formalin-fixed, paraffin-embedded human Alzheimer disease brain tissue. Following antigen retrieval using Sodium Citrate H.E.I.R., the tissue was incubated with Biotin-conjugated antibody at 20 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection, followed by hematoxylin counterstaining.
  • 8E10dotD9_Biotin_CX3CR1_Antibody_IHC-P_010417
    IHC staining of anti-CX3CR1 antibody (clone 8E10.D9) conjugated to biotin on formalin-fixed, paraffin-embedded human Alzheimer disease brain tissue. Following antigen retrieval using Sodium Citrate H.E.I.R., the tissue was incubated with Biotin-conjugated antibody at 20 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection, followed by hematoxylin counterstaining.
See high resolution IHC-P data...
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824004 100 µg 300 CHF
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Description

CX3C chemokine receptor 1 (CX3CR1) also known as fractalkine receptor or G-protein coupled receptor 13, is a receptor that binds the chemokine CX3CL1. CX3CR1 expression is associated with T cells, NK cells, and brain microglia. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediates cell adhesion and migration. CX3CL1 has been reported to play an important role in neuroinflammation and neurodegeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 207-222 of the human CX3CR1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 20 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in Immunoblotting (WB) and immunohistochemistry (IHC) for the relevant formats of this clone.

RRID
AB_2650684 (BioLegend Cat. No. 824004)

Antigen Details

Structure
CX3CR1 is a 355 amino acid G-protein coupled receptor, 7 transmembrane regions protein with a molecular mass of 40 kD.
Distribution

Tissue Distribution: central nervous system, and blood.
Cellular Distribution: plasma membrane.

Function
CX3CR1 is involved in cell recruitment during inflammation, and participates in cell adhesion and extravasation from blood vessels. It is also known as a coreceptor for HIV1.
Ligand/Receptor
CX3CL1 (fractalkine, neurotactin).
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Chen P, et al. 2016. BioMed. Res. Int. 2016. 8090918. PubMed
2. Sheridan GK, Murphy KJ. 2013. Open Biol. 3 (12) 130181. PubMed

Gene ID
1524 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 01.16.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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