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Biotin anti-human CD215 (IL-15Rα) Antibody

Pricing & Availability
Clone
JM7A4 (See other available formats)
Regulatory Status
RUO
Other Names
IL-15Rα chain, IL-15R, IL-15Rα
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
JM7A4_Biotin_071608.jpg
IFN-γ + LPS-stimulated human peripheral blood monocytes stained with biotinylated JM7A4, followed by Sav-PE.
  • JM7A4_Biotin_071608.jpg
    IFN-γ + LPS-stimulated human peripheral blood monocytes stained with biotinylated JM7A4, followed by Sav-PE.
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330204 100 µg 265 CHF
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Description

IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8+ T cell proliferation, and NK/NKT cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Vamosi G, et al. 2004. P. Natl. Acad. Sci. USA. 101:11082.
  2. Dubois S, et al. 2002. Immunity 17:537.
  3. Veerman KM, et al. 2012. J. Immunol. 188:1638. (FC) PubMed
  4. Vujanovic L, et al. 2010. Blood. 116:575. (FC) PubMed
  5. Kitaya K, et al. 2013. Biol. Reprod. 89:70. (FC) PubMed
RRID
AB_2248982 (BioLegend Cat. No. 330204)

Antigen Details

Distribution

Dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells

Function
Support T and B cell proliferation and development, preferentially for memory CD8+ T cell proliferation, and NK/NKT cell development
Ligand/Receptor
IL-15 with high affinity
Cell Type
Dendritic cells, Monocytes, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Giri JG, et al. 1995. EMBO J. 14:3654.
2. Giron-Michel J, et al. 2005. Blood 106:2302.
3. Lorenzen I, et al. 2005. J. Biol. Chem. 281:6642.
4. Sato N, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:588.

Gene ID
3601 View all products for this Gene ID
UniProt
View information about CD215 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 06.02.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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