Loading...

Biotin anti-human CXCL13 Antibody

Pricing & Availability
Clone
A15151H (See other available formats)
Regulatory Status
RUO
Other Names
B cell attracting chemokine (BCA-1)
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A15151H_Biotin_CXCL13_Antibody_051023
Direct ELISA showing biotin anti-human CXCL13 (clone A15151H) bound to plate-immobilized recombinant human CXCL13 protein (Cat. Nos. 574702, 574704, 574706 and 574708). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A15151H_Biotin_CXCL13_Antibody_051023
    Direct ELISA showing biotin anti-human CXCL13 (clone A15151H) bound to plate-immobilized recombinant human CXCL13 protein (Cat. Nos. 574702, 574704, 574706 and 574708). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
Cat # Size Price Quantity Check Availability Save
612653 50 µg 235 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CXCL13 was initially described as a chemokine highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells in B cell areas of secondary lymphoid tissue. CXCR5, the receptor for CXCL13, was initially cloned from Burkitt’s lymphoma cells and named BLR1. CXCR5 is highly expressed in those cells and B cells. CXCL13, CCL19, and CCL21 are involved in the formation of tertiary lymphoid tissues in chronic antigeninduced arthritis. CXCL13 takes part in the allergic airway inflammatory process. In addition, high expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, and rheumatoid arthritis. In summary, CXCL13 is highly expressed at the sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant CXCL13 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15151H) could function as a detection antibody when paired with purified anti-human CXC13 antibody (Cat. No. 613352) (clone A15151A) as the capture antibody and using Recombinant Human CXCL13 protein (Cat. Nos. 574702, 574704, 574706, and 574708) as the assay standard.

RRID
AB_2941608 (BioLegend Cat. No. 612653)

Antigen Details

Structure
Chemokine
Distribution

Follicular dendritic cells, monocytes, macrophages, and stromal cells from germinal centers. CXCL13 is derived from endothelial cells, synovial fibroblasts, and follicular dendritic cells in RA.

Function
CXCL13 chemoattracts B cells and is a key chemokine for B-cell entry into lymphoid follicles. It also takes part in the formation of ectopic lymphoid tissue in chronic inflammation. CXCL13 production by follicular Th cells is induced by TCR activation.
Interaction
B cells and follicular helper CD4 T cells
Ligand/Receptor
CXCR5 (CD185)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Gunn MD, et al. 1998. Nature. 391:799-803.
  2. Legler DF, et al. 1998. J Exp Med. 187:655-60.
  3. Kim CH, et al. 2004. Blood. 104:1952-60.
  4. McDonald KG, et al. 2010. Am J Pathol. 176:2367.
  5. Gatto D, et al. 2011. J Immunol. 187:4621-8.
Gene ID
10563 View all products for this Gene ID
UniProt
View information about CXCL13 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 04.24.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account