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Purified anti-human CXCL13 Antibody

Pricing & Availability
Clone
A15151H (See other available formats)
Regulatory Status
RUO
Other Names
B cell attracting chemokine (BCA-1)
Isotype
Mouse IgG2a, κ
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Product Citations
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A15151H_PURE_-CXCL13_Antibody_050323
Direct ELISA showing purified anti-human CXCL13 (clone A15151H) bound to plate-immobilized recombinant human CXCL13 protein (Cat. Nos. 574702, 574704, 574706, and 574708). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSAPBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A15151H_PURE_-CXCL13_Antibody_050323
    Direct ELISA showing purified anti-human CXCL13 (clone A15151H) bound to plate-immobilized recombinant human CXCL13 protein (Cat. Nos. 574702, 574704, 574706, and 574708). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSAPBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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612652 100 µg 465 CHF
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Description

CXCL13 was initially described as a chemokine highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells in B cell areas of secondary lymphoid tissue. CXCR5, the receptor for CXCL13, was initially cloned from Burkitt’s lymphoma cells and named BLR1. CXCR5 is highly expressed in those cells and B cells. CXCL13, CCL19, and CCL21 are involved in the formation of tertiary lymphoid tissues in chronic antigeninduced arthritis. CXCL13 takes part in the allergic airway inflammatory process. In addition, high expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, and rheumatoid arthritis. In summary, CXCL13 is highly expressed at the sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant CXCL13 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15151H) could function as a detection antibody when paired with purified anti-human CXCL13 antibody (Cat. No. 613352) (clone A15151A) as the capture antibody and using Recombinant Human CXCL13 protein (Cat. Nos. 574702, 574704, 574706, and 574708) as the assay standard. For assay development flexibility purpose this product is provided as purified format. 

Antigen Details

Structure
Chemokine
Distribution

Follicular dendritic cells, monocytes, macrophages, and stromal cells from germinal centers. CXCL13 is derived from endothelial cells, synovial fibroblasts, and follicular dendritic cells in RA.

Function
CXCL13 chemoattracts B cells and is a key chemokine for B-cell entry into lymphoid follicles. It also takes part in the formation of ectopic lymphoid tissue in chronic inflammation. CXCL13 production by follicular Th cells is induced by TCR activation
Interaction
B cells and follicular helper CD4 T cells.
Ligand/Receptor
CXCR5 (CD185)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Gunn MD, et al. 1998. Nature. 391:799-803.
  2. Legler DF, et al. 1998. J Exp Med. 187:655-60.
  3. Kim CH, et al. 2004. Blood. 104:1952-60. 
  4. McDonald KG, et al. 2010. Am J Pathol. 176:2367.
  5. Gatto D, et al. 2011. J Immunol. 187:4621-8.
Gene ID
10563 View all products for this Gene ID
UniProt
View information about CXCL13 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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