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Biotin anti-human DR3 (TRAMP) Antibody

Pricing & Availability
Clone
JD3 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF25, TRAMP, Apo-3, WSL-1, LARD, DDR3, TR3, TNFRSF12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
JD3_Biotin_070808.jpg
Human Tramp transfected cells stained with biotinylated JD3, followed by Sav-PE
  • JD3_Biotin_070808.jpg
    Human Tramp transfected cells stained with biotinylated JD3, followed by Sav-PE
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307104 100 µg 265 CHF
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Description

DR3 is also known as TNFRSF25, Apo-3, WSL-1, TRAMP, LARD, DDR3, and TR3. It is a member of the TNF receptor family expressed at low level by monocytes and granulocytes. Interaction of DR3 with its ligand (TWEAK), activates the NF-κB pathway and induces apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
human DR3-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: The JD3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR3/TRAMP receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307104) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2002. J. Immunol. 168:734.
RRID
AB_2207081 (BioLegend Cat. No. 307104)

Antigen Details

Structure
TNFR superfamily
Distribution

Activated lymphocytes and thymocytes

Function
Lymphocyte homeostasis
Ligand/Receptor
Apo-3L, TWEAK
Cell Type
Lymphocytes, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Nakayama M, et al. 2002. J. Immunol. 168:734.
2. Nakayama M, et al. 2000. J. Exp. Med. 192:1373.

Gene ID
8718 View all products for this Gene ID
UniProt
View information about DR3 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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