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PE anti-human DR3 (TRAMP) Antibody

Pricing & Availability
Clone
JD3 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF25, TRAMP, Apo-3, WSL-1, LARD, DDR3, TR3, TNFRSF12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
JD3_PE_DR3_Antibody_1_052924
Human TRAMP transfected cells were stained with anti-human DR3 (TRAMP) (clone JD3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • JD3_PE_DR3_Antibody_1_052924
    Human TRAMP transfected cells were stained with anti-human DR3 (TRAMP) (clone JD3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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307105 25 tests 141 CHF
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307106 100 tests 304 CHF
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Description

DR3 is also known as TNFRSF25, Apo-3, WSL-1, TRAMP, LARD, DDR3, and TR3. It is a member of the TNF receptor family expressed at low level by monocytes and granulocytes. Interaction of DR3 with its ligand (TWEAK), activates the NF-κB pathway and induces apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
human DR3-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The JD3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR3/TRAMP receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307104) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2002. J. Immunol. 168:734.
Product Citations
  1. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  2. Cavallini C, et al. 2013. PLoS One. 8:60136. PubMed
RRID
AB_2240863 (BioLegend Cat. No. 307105)
AB_2240863 (BioLegend Cat. No. 307106)

Antigen Details

Structure
TNFR superfamily
Distribution

Activated lymphocytes and thymocytes

Function
Lymphocyte homeostasis
Ligand/Receptor
Apo-3L, TWEAK
Cell Type
Lymphocytes, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Nakayama M, et al. 2002. J. Immunol. 168:734.
2. Nakayama M, et al. 2000. J. Exp. Med. 192:1373.

Gene ID
8718 View all products for this Gene ID
UniProt
View information about DR3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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