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Biotin anti-mouse Lineage Panel

Pricing & Availability
Clone
145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
RUO
Other Names
Lin-1
Isotype
Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
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Product Citations
publications
MU_Biotin_Lineage_Cocktail_012712
C57BL/6 mouse bone marrow cells were stained with Biotin Lineage Cocktail, followed by Sav-FITC and CD117 APC.
  • MU_Biotin_Lineage_Cocktail_012712
    C57BL/6 mouse bone marrow cells were stained with Biotin Lineage Cocktail, followed by Sav-FITC and CD117 APC.
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133307 500 tests 323 CHF
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. This product includes individual vials of the following biotin antibodies: anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid Cells, clone TER-119. 

Materials provided:
1. 500 tests (1 mL) each of: Biotin anti-mouse CD3ε, Biotin anti-mouse Ly-6G/Ly-6C (Gr-1), Biotin anti-mouse CD11b, Biotin anti-mouse CD45R/B220, Biotin anti-mouse Ter-119/Erythroid Cells

Materials not provided:

  1. Cell Staining Buffer (Cat. No. 420201)
  2. Isotype control reagents (Armenian hamster IgG, Rat IgG2a, Rat IgG2b)
Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solutions should be stored undiluted at 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Magnetic particle separation - Verified
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2 µl of each antibody per million cells or 2 µl of each antibody per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
  2. Okada S, et al. 1992. Blood180 (12):3044-3050
  3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
  4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
  5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
  6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations
  1. Chudakov DB, et al. 2022. Vaccines (Basel). 10: . PubMed
  2. Li X, et al. 2022. Cell. 185:1709. PubMed
  3. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  4. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  5. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  6. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
  7. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  8. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  9. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
  10. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  11. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  12. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  13. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
  14. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  15. Freter R, et al. 2021. Nat Commun. 12:3318. PubMed
  16. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  17. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  18. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  19. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  20. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  21. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  22. Guo L, et al. 2020. Nat Commun. 0.972222222. PubMed
  23. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  24. Lion M, et al. 2020. Proc Natl Acad Sci U S A. 117:5453. PubMed
RRID
AB_11124348 (BioLegend Cat. No. 133307)

Antigen Details

Distribution

T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes

Biology Area
Immunology
Antigen References

1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Lineage Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
PE anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Biotin anti-mouse Lineage Panel 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Go To Top Version: 3    Revision Date: 05.16.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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