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FITC anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
Clone
145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
RUO
Other Names
Lin-1
Isotype
Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
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Product Citations
publications
A_FITC_Lineage_042909.jpg
C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 (clone RAM34) Alexa Fluor® 647
  • A_FITC_Lineage_042909.jpg
    C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 (clone RAM34) Alexa Fluor® 647
  • B_FITC_Lineage_042909.jpg
    C57BL/6 bone marrow cells stained with FITC isotype control cocktail and CD34 (clone RAM34) Alexa Fluor® 647
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133301 25 tests 382 CHF
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133302 100 tests 645 CHF
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. FITC anti-mCD3ε/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 tests
2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 tests


Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Formulation
This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation
The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
  2. Okada S, et al. 1992. Blood180 (12):3044-3050
  3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
  4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
  5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
  6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations
  1. Zhang S, et al. 2022. Mol Carcinog. 61:408. PubMed
  2. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  3. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  4. Broxmeyer HE, et al. 2021. Blood Cells Mol Dis. 86:102492. PubMed
  5. Broxmeyer HE, et al. 2022. Stem Cell Rev Rep. 18:2513. PubMed
  6. Li X, et al. 2022. Cell. 185:1709. PubMed
  7. Sakae H, et al. 2023. Cells. 12:. PubMed
  8. Guan H, et al. 2023. Int J Mol Sci. 24:. PubMed
  9. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  10. Tikka C, et al. 2023. Front Immunol. 14:1026368. PubMed
  11. Feng X, et al. 2023. Nat Commun. 14:3208. PubMed
  12. Cai Q, et al. 2018. Blood Cells Mol Dis. 71:23. PubMed
  13. Chen G, et al. 2014. Cancer Immunol Res. 2:949. PubMed
  14. Xue Y et al. 2017. Developmental cell. 42(4):349-362 . PubMed
  15. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  16. Capitano ML, et al. 2020. Blood Cells Mol Dis. 102435:84. PubMed
  17. Liu J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.010. PubMed
  18. Gao S, et al. 2022. Cell Res. 32:38. PubMed
  19. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  20. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
  21. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  22. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  23. Capitano ML, et al. 2019. J Clin Invest. 130:2555. PubMed
  24. Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed
  25. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  26. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  27. Yu Q, et al. 2016. Cell Res. 10.1038/cr.2016.85. PubMed
  28. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  29. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  30. Capitano ML, et al. 2021. J Clin Invest. 131:. PubMed
  31. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  32. Liu X, et al. 2015. Nat Commun. 6: 8825. PubMed
RRID
AB_10697030 (BioLegend Cat. No. 133301)
AB_10697030 (BioLegend Cat. No. 133302)

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Other Formats

View All Lineage Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
PE anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Biotin anti-mouse Lineage Panel 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Go To Top Version: 2    Revision Date: 07.09.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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