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Biotin anti-Tau, 189-195 Antibody

Pricing & Availability
Clone
39E10 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein tau, PHF-tau, Paired Helical Filament tau
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
39E10_Biotin_Tau_189-195_Antibody_1_WB_010517
Western blot of biotin anti-Tau, 189-195 antibody (clone 39E10) and isotype-matched control IgG1. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of rat brain lysate; Lane 3: 20 µg of mouse brain lysate. The blots were treated with biotin block, incubated for two hours at room temperature with 5 µg/mL of the primary antibody or mouse IgG1, followed by incubation with Streptavidin-HRP (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • 39E10_Biotin_Tau_189-195_Antibody_1_WB_010517
    Western blot of biotin anti-Tau, 189-195 antibody (clone 39E10) and isotype-matched control IgG1. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of rat brain lysate; Lane 3: 20 µg of mouse brain lysate. The blots were treated with biotin block, incubated for two hours at room temperature with 5 µg/mL of the primary antibody or mouse IgG1, followed by incubation with Streptavidin-HRP (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • 39E10_Biotin_Tau_189-195_Antibody_2_ELISA_010917
    Direct ELISA of biotin anti-Tau, 189-195 antibody (clone 39E10) binding to plate-immobilized recombinant human 2N3R and 2N4R tau proteins. ELISA was performed by coating wells with 150 ng of each recombinant tau protein for two hours at 37°C. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled HRP goat anti-mouse IgG antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system
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814303 25 µg 124 CHF
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814304 100 µg 300 CHF
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against full-length human tau protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 µg per ml.  For Direct ELISA, the suggested use of this reagent is 10 ng to 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

BioLegend is the Exclusive Provider of this Antibody Product

Application References

(PubMed link indicates BioLegend citation)
  1. Michalski V, et.al. Neurobiol Aging. 2015. 36(12):3131. (WB) PubMed
RRID
AB_2650699 (BioLegend Cat. No. 814303)
AB_2650699 (BioLegend Cat. No. 814304)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with: Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References

1. Meredith JE Jr, et al. 2013. PLoS One. 8(10): e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1): 89. PubMed
3. Wang Y, Mandelkow E. 2016. Nat. Rev. Neurosci. 17(1):5-21. PubMed

Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau 189-195 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 01.29.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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