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Brilliant Violet 421™ anti-human CD158e1 (KIR3DL1, NKB1) Antibody

Pricing & Availability
Clone
DX9 (See other available formats)
Regulatory Status
RUO
Other Names
NKAT3, NKB1, KIR-NKB1, p70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DX9_BV421_1_052611
Human peripheral blood lymphocytes were stained with CD56 APC and CD158e1 (clone DX9) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom).
  • DX9_BV421_1_052611
    Human peripheral blood lymphocytes were stained with CD56 APC and CD158e1 (clone DX9) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom).
  • DX9_BV421_2_052611
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
312713 25 tests 193 CHF
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312714 100 tests 402 CHF
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Description

CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell clone VL186-1.6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312710).

Application References

(PubMed link indicates BioLegend citation)
  1. Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
  2. Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
  3. Gardiner CM, et al. 2001. J. Immunol. 166:2992.
  4. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
  5. Goodier M, et al. 2000. J. Immunol. 165:139.
  6. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
  7. Yawata M, et al. 2002. Immunogenetics 54:543.
  8. Valiante NM, et al. 1997. Immunity 7:739.
  9. Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
  10. Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
  11. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  12. Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
Product Citations
  1. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  2. Marquardt N, et al. 2015. J Immunol. 195: 3262 - 3272. PubMed
  3. Jo DH, et al. 2023. Mol Ther Methods Clin Dev. 29:173. PubMed
  4. Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed
  5. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  6. Lisovsky I, et al. 2015. J Virol. 89: 9909 - 9919. PubMed
  7. Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
  8. Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
  9. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  10. Lisovsky I, et al. 2015. J Leukoc Biol. 97:761. PubMed
  11. Isitman G, et al. 2016. PLoS One. 11:e0164517. PubMed
  12. Boudreau J, et al. 2014. PLoS One. 9:99543. PubMed
  13. Terszowski G, et al. 2014. J Immunol. 192:5618. PubMed
  14. Purdy A, et al. 2014. J Immunol. 193:4675. PubMed
RRID
AB_2561652 (BioLegend Cat. No. 312713)
AB_2561652 (BioLegend Cat. No. 312714)

Antigen Details

Structure
Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
Distribution

Subset of NK and T cells

Function
Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
Ligand/Receptor
HLA-B antigens such as the HLA-Bw4 allele
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.

Gene ID
3811 View all products for this Gene ID
UniProt
View information about CD158e1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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