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- Clone
- A20153E (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) APC and anti-human CD7 (clone A20153E) Brilliant Violet 421™ (left) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 385811 | 25 tests | 165 CHF | ||||
| 385812 | 100 tests | 400 CHF | ||||
CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD7 recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Clone A20153E can completely block binding of clone 4H9-CD7 and partially blocks binding of clone CD7-6B7 on target cells.
- RRID
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AB_3662284 (BioLegend Cat. No. 385811)
AB_3662284 (BioLegend Cat. No. 385812)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 40 kD
- Distribution
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T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets
- Function
- T cell Activation
- Cell Type
- Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Stillwell R, et al. 2001. Immunol Res. 24:31-52.
- Rabinowich H, et al. 1994. J Immunol. 152:517-26.
- Gene ID
- 924 View all products for this Gene ID
- UniProt
- View information about CD7 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD7 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD7 | A20153E | FC |
| PE anti-human CD7 | A20153E | FC |
| PE/Dazzle™ 594 anti-human CD7 | A20153E | FC |
| Alexa Fluor® 488 anti-human CD7 | A20153E | FC |
| APC anti-human CD7 | A20153E | FC |
| Brilliant Violet 421™ anti-human CD7 | A20153E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD7
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD7
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Dazzle™ 594 anti-human CD7
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 488 anti-human CD7
Human peripheral blood lymphocytes were stained with anti-h... -
APC anti-human CD7
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD7
Human peripheral blood lymphocytes were stained with anti-hu...
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