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Brilliant Violet 421™ anti-mouse CD209a (DC-SIGN) Antibody

Pricing & Availability
Clone
MMD3 (See other available formats)
Regulatory Status
RUO
Other Names
CD209 antigen-like protein A, dendritic cell-specific ICAM-3-grabbing non-integrin, CIRE, CD209, CDSIGN
Isotype
Mouse IgG2c, κ
Ave. Rating
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Product Citations
publications
MMD3_BV421_CD209a_Antibody_041923
CHO-DC-SIGN transfected cells were stained with anti-mouse CD209a (DC-SIGN) (clone MMD3) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • MMD3_BV421_CD209a_Antibody_041923
    CHO-DC-SIGN transfected cells were stained with anti-mouse CD209a (DC-SIGN) (clone MMD3) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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833007 50 µg 270 CHF
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Description

CD209a, is also known as dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and CIRE. Of the eight DC-SIGN-related receptors in mice, CD209a is considered the closest homolog to human DC-SIGN (CD209). Although both mouse and human counterparts are expressed on monocyte-derived dendritic cells, expression variation has been noted. Mouse CD209a is found on skin and splenic myeloid cells and is highly expressed on plasmacytoid dendritic cells, while human CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. It plays a critical role in capturing and internalizing pathogens such as Streptococcus pneumoniae.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a purified extracellular domain of mouse CD209a (DC-SIGN)-IgG1 Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The antibody will bind to both C57 and Balb/C mouse tissues.

RRID
AB_2941634 (BioLegend Cat. No. 833007)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Cai M, et al. 2016. Clin. Exp. Immunol. 317-25.
2. Ponichtera HE, et al. 2014. J. Immunol. 4655-65.

Gene ID
170786 View all products for this Gene ID
UniProt
View information about CD209a on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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