Brilliant Violet 650™ anti-human CD134 (OX40) Antibody, CD134, Ber-ACT35 (ACT35)

Pricing & Availability

Clone: Ber-ACT35 (ACT35) (See other available formats)
Regulatory Status: RUO
Other Names: ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype: Mouse IgG1, κ
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Product Citations: publications

Ber-ACT35_BV650_CD134_Antibody_071224 — PHA-stimulated (3-days) human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD134 (OX40) (clone Ber-ACT35) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ isotype control (right).

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350049

25 tests

205 CHF

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350050

100 tests

420 CHF

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Description

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4⁺ T cell tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV 1-transformed HUT 102 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, immunohistochemical staining^2,3of paraffin embedded⁷ and frozen tissue sections, ELISA⁴, and functional assay⁵. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
RRID: AB_3662250 (BioLegend Cat. No. 350049)
AB_3662250 (BioLegend Cat. No. 350050)

Antigen Details

Structure: 50 kD, TNF receptor family
Distribution: Activated T cells
Function: Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor: OX40L (CD252)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581.
Gene ID: 7293 View all products for this Gene ID
UniProt: View information about CD134 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD134 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Purified anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC,IHC-P,ELISA,IHC-F,WB
PE anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PerCP/Cyanine5.5 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Cyanine7 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 421™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Purified anti-human CD134 (OX40) (Maxpar® Ready)	Ber-ACT35 (ACT35)	FC,CyTOF®
Alexa Fluor® 647 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Dazzle™ 594 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC/Cyanine7 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Biotin anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 510™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 605™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC/Fire™ 750 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 711™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
TotalSeq™-A0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-C0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-B0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-D0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
PE/Cyanine5 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Fire™ 810 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE anti-human CD134	Ber-ACT35 (ACT35)	FC
PE/Fire™ 744 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 785™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 650™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.12.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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