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PE/Fire™ 810 anti-human CD134 (OX40) Antibody

Pricing & Availability
Clone
Ber-ACT35 (ACT35) (See other available formats)
Regulatory Status
RUO
Other Names
ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Ber-ACT35_PEFire810_CD134_OX40_Antibody_041922
Human peripheral blood lymphocytes were activated for 3 days with PHA, and then stained with anti-human CD3 FITC and anti-human OX-40 (clone Ber-ACT35) PE/Fire™ 810 (left) or stained with anti-human CD3 FITC (right) only.
  • Ber-ACT35_PEFire810_CD134_OX40_Antibody_041922
    Human peripheral blood lymphocytes were activated for 3 days with PHA, and then stained with anti-human CD3 FITC and anti-human OX-40 (clone Ber-ACT35) PE/Fire™ 810 (left) or stained with anti-human CD3 FITC (right) only.
Compare all formats See PE/Fire™ 810 spectral data
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350043 25 tests 204 CHF
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Description

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4+ T cell tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV 1-transformed HUT 102 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

 

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
  2. Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
  3. Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
  4. Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
  5. Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
  6. Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
  7. Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
RRID
AB_2924549 (BioLegend Cat. No. 350043)

Antigen Details

Structure
50 kD, TNF receptor family
Distribution

Activated T cells

Function
Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor
OX40L (CD252)
Cell Type
T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581.

Gene ID
7293 View all products for this Gene ID
UniProt
View information about CD134 on UniProt.org

Other Formats

View All CD134 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Purified anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC,IHC-P,ELISA,IHC-F,WB
PE anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
APC anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
PerCP/Cyanine5.5 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
PE/Cyanine7 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Brilliant Violet 421™ anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Purified anti-human CD134 (OX40) (Maxpar® Ready) Ber-ACT35 (ACT35) FC,CyTOF®
Alexa Fluor® 647 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
PE/Dazzle™ 594 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
APC/Cyanine7 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Biotin anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Brilliant Violet 510™ anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Brilliant Violet 605™ anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
APC/Fire™ 750 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Brilliant Violet 711™ anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
TotalSeq™-A0158 anti-human CD134 (OX40) Ber-ACT35 (ACT35) PG
TotalSeq™-C0158 anti-human CD134 (OX40) Ber-ACT35 (ACT35) PG
TotalSeq™-B0158 anti-human CD134 (OX40) Ber-ACT35 (ACT35) PG
TotalSeq™-D0158 anti-human CD134 (OX40) Ber-ACT35 (ACT35) PG
PE/Cyanine5 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
PE/Fire™ 810 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
PE anti-human CD134 Ber-ACT35 (ACT35) FC
PE/Fire™ 744 anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Brilliant Violet 785™ anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Brilliant Violet 650™ anti-human CD134 (OX40) Ber-ACT35 (ACT35) FC
Go To Top Version: 1    Revision Date: 04.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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