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Brilliant Violet 785™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
30-F11_BV785_CD45_Antibody_FC_121014
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 785™ (filled histogram) or rat IgG2b, κ Brilliant Violet 785™ isotype control (open histogram).
  • 30-F11_BV785_CD45_Antibody_FC_121014
    C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 785™ (filled histogram) or rat IgG2b, κ Brilliant Violet 785™ isotype control (open histogram).
Compare all formats See Brilliant Violet 785™ spectral data
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103149 50 µg 273 CHF
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  2. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  3. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  4. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  5. Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed
  6. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  7. Wu J, et al. 2023. Bioact Mater. 20:259. PubMed
  8. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  9. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
  10. Hill AA, et al. 2023. JCI Insight. 8: . PubMed
  11. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  12. Lazure F, et al. 2023. Nat Commun. 14:535. PubMed
  13. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  14. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  15. Jia L, et al. 2022. Gut Microbes. 14:2117503. PubMed
  16. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  17. Jeon Y, et al. 2022. Cancers (Basel). 14:. PubMed
  18. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  19. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  20. Prasad S, et al. 2023. iScience. 26:106628. PubMed
  21. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  22. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  23. Myers JA, et al. 2022. JCI Insight. :. PubMed
  24. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  25. Rogic A, et al. 2021. Nat Commun. 12:6889. PubMed
  26. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  27. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  28. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  29. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  30. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  31. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  32. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  33. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  34. Burns JC, et al. 2021. Bio Protoc. 11:e4091. PubMed
  35. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  36. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  37. Johnson LA, et al. 2021. Life Sci Alliance. :4. PubMed
  38. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  39. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  40. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  41. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  42. Burns JC, et al. 2020. eLife. 9:00. PubMed
  43. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  44. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  45. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  46. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  47. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  48. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  49. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  50. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  51. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  52. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  53. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  54. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  55. Chen B, et al. 2021. Cell. 184:6262. PubMed
  56. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  57. Lee HN, et al. 2022. JCI Insight. 7:. PubMed
  58. Krisna SS, et al. 2021. STAR Protoc. 2:100627. PubMed
  59. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
  60. Deets KA, et al. 2021. Elife. 10:. PubMed
  61. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_2564590 (BioLegend Cat. No. 103149)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 1    Revision Date: 12.12.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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