Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody, 5.1H11

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Clone: 5.1H11 (See other available formats)
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ
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5.1H11_CV_GMP_ULEAF_BIOTIN_CD56_111423 — Human peripheral leukocytes were stained with Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF antibody (clone 5.1H11, filled histogram) or isotype control (clone MOPC-21, open histogram) followed by PE Streptavidin stain.

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362574

1 mg

2790 CHF

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362573

100 µg

578 CHF

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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronalderived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody contains ultra-low levels of endotoxin (<0.01 EU/μg of protein), is filtered through a 0.1 μm membrane, and is tested negative for mycoplasma and microbial growths.

Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody is for research and further ex vivo cell processing use only.

Product Details

Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

- Ultra-Low endotoxin level (<0.01 EU/μg)

- Manufactured under serum-free conditions

- Bulks tested negative for mycoplasma and bacterial/fungal growth

- Serum-free hybridomas are negative for the presence of murine virus

- Batch-to-batch consistency

- Vendor qualification

- Raw material traceability and documentation

- Documented procedures and employee training

- Equipment maintenance and monitoring records

- Lot-specific certificates of analysis

- QA review of released products

- Quality audits per ISO 13485:2016

Technical Data Sheet (pdf)

Product Details

Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myotube cells
Formulation: 0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Endotoxin Level: < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: Serum-Free hybridoma derived Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody purified by affinity chromatography
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research or further ex vivo cell processing use only.

Antigen Details

Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL, and myeloid leukemias
Function: adhesion
Ligand/Receptor: Heparan sulfate
Antigen References: Lanier L, et al. 1991. J. Immunol. 146:4421

Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71

Cremer H, et al. 1994. Nature. 367:455.
Gene ID: 4684 View all products for this Gene ID
UniProt: View information about CD56 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD56 (NCAM)	5.1H11	FC
APC anti-human CD56 (NCAM)	5.1H11	FC
PerCP/Cyanine5.5 anti-human CD56 (NCAM)	5.1H11	FC
PE anti-human CD56 (NCAM)	5.1H11	FC
PE/Cyanine7 anti-human CD56 (NCAM)	5.1H11	FC
APC/Cyanine7 anti-human CD56 (NCAM)	5.1H11	FC
PE/Cyanine5 anti-human CD56 (NCAM)	5.1H11	FC
Alexa Fluor® 647 anti-human CD56 (NCAM)	5.1H11	FC
Alexa Fluor® 488 anti-human CD56 (NCAM)	5.1H11	FC
Pacific Blue™ anti-human CD56 (NCAM)	5.1H11	FC
Alexa Fluor® 700 anti-human CD56 (NCAM)	5.1H11	FC
PerCP anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 650™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 510™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 421™ anti-human CD56 (NCAM)	5.1H11	FC
Biotin anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 605™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 570™ anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 711™ anti-human CD56 (NCAM)	5.1H11	FC
PE/Dazzle™ 594 anti-human CD56 (NCAM)	5.1H11	FC
FITC anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 785™ anti-human CD56 (NCAM)	5.1H11	FC
Ultra-LEAF™ Purified anti-human CD56 (NCAM)	5.1H11	FC
APC/Fire™ 750 anti-human CD56 (NCAM)	5.1H11	FC
PE anti-human CD56 (NCAM)	5.1H11	FC
APC anti-human CD56 (NCAM)	5.1H11	FC
Brilliant Violet 750™ anti-human CD56 (NCAM)	5.1H11	FC
TotalSeq™-A0047 anti-human CD56 (NCAM)	5.1H11	PG
TotalSeq™-C0047 anti-human CD56 (NCAM)	5.1H11	PG
TotalSeq™-B0047 anti-human CD56 (NCAM)	5.1H11	PG
Spark NIR™ 685 anti-human CD56 (NCAM)	5.1H11	FC
KIRAVIA Blue 520™ anti-human CD56 (NCAM)	5.1H11	FC
GMP PE anti-human CD56 (NCAM)	5.1H11	FC
TotalSeq™-D0047 anti-human CD56 (NCAM)	5.1H11	PG
GMP APC anti-human CD56 (NCAM)	5.1H11	FC
Spark YG™ 593 anti-human CD56 (NCAM)	5.1H11	FC
Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD56 (NCAM) SF	5.1H11	FC
Spark Red™ 718 anti-human CD56 (NCAM)	5.1H11	FC
Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD56 (NCAM) SF	5.1H11	FC
APC/Fire™ 810 anti-human CD56 (NCAM) Antibody	5.1H11	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.27.2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.